Effects of a SARS-associated coronavirus vaccine in monkeys.
Effects of a SARS-associated coronavirus vaccine in monkeys.
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DOI：
10.1016/s0140-6736(03)14962-8
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发表时间：
2003-12-06
期刊：
影响因子：
--
通讯作者：
Gambotto A
中科院分区：
文献类型：
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作者：
Gao W;Tamin A;Soloff A;D'Aiuto L;Nwanegbo E;Robbins PD;Bellini WJ;Barratt-Boyes S;Gambotto A
严重急性呼吸系统综合症（SARS）的病原体已被确定为一种新型冠状病毒。在这里，我们已经调查了密码子优化的SARS-CoV株Urbani结构抗原刺突蛋白S1片段，膜蛋白和核衣壳蛋白在恒河猴中诱导病毒特异性广泛免疫的能力。我们用三种Ad 5-SARS-CoV载体或对照载体的组合肌肉内免疫恒河猴，并在第28天给予加强免疫。接种疫苗的动物均具有针对刺突蛋白S1片段的抗体应答和针对核衣壳蛋白的T细胞应答。所有接种疫苗的动物在体外对SARS-CoV感染均表现出强烈的中和抗体应答。这些结果表明，基于腺病毒的疫苗可以在猴子中诱导强烈的SARS-CoV特异性免疫应答，并有望开发针对SARS病原体的保护性疫苗。
The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.