Synthesis of virus DNA and polypeptides by temperature-sensitive mutants of rabbitpox virus.
Synthesis of virus DNA and polypeptides by temperature-sensitive mutants of rabbitpox virus.
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兔痘病毒温度敏感突变体合成病毒DNA和多肽。
DOI：
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发表时间：
1980
影响因子：
3.8
通讯作者：
P. Cooper
中科院分区：
文献类型：
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作者：
J. Lake;P. Cooper
对18个温度敏感的兔痘病毒突变体进行了DNA和蛋白质合成缺陷的检测。两个突变体（ts-3和ts-16）在DNA合成（DNA-）方面存在缺陷，因为当感染细胞在限制性温度下孵育时，它们将明显少于野生型的标记胸腺嘧啶掺入酸沉淀物质中。在40℃孵育后，用SDS（聚丙烯酰胺平板凝胶电泳）检测感染细胞提取物时，这两个突变体都只产生了“早期”类病毒多肽。其余16个DNA+ ts突变体中的9个（ts-1、ts-2、ts-6、ts-12、ts-15、ts-17、ts-31、ts-32和ts-33）在40℃下合成了大多数病毒多肽的野生型水平；6个DNA+ ts突变体（ts-7、ts-8、ts-9、ts-11、ts-23和ts-24）在参与膜稳定和颗粒组装的多肽的翻译后切割中存在缺陷；一个DNA+ ts突变体（ts-14）只合成了“早期”类病毒多肽，这意味着要么复制的DNA没有完全发挥功能，要么需要一种特定的早期功能来进行后期转录。
Eighteen temperature-sensitive (ts) mutants of rabbitpox virus were examined for defects in synthesis of DNA and protein. Two mutants (ts-3 and ts-16) were defective in DNA synthesis (DNA-), since both incorporated significantly less than wild-type amounts of labelled thymidine into acid-precipitable material when infected cells were incubated at the restrictive temperature. Both these mutants gave only the 'early' class of virus polypeptides when infected cell extracts were examined by SDS--polyacrylamide slab gel electrophoresis following incubation at 40 degrees C. Nine of the remaining sixteen DNA+ ts mutants (ts-1, ts-2, ts-6, ts-12, ts-15, ts-17, ts-31, ts-32 and ts-33) synthesized wild-type levels of most virus polypeptides at 40 degrees C; six DNA+ ts mutants (ts-7, ts-8, ts-9, ts-11, ts-23 and ts-24) were defective in the post-translational cleavage of the polypeptides involved in membrane stabilization and particle assembly; one DNA+ ts mutant (ts-14) synthesized only the 'early' class of virus polypeptides, implying that either replicated DNA was not fully functional or that a specific early function was required for late transcription.