Capturing and profiling adult hair follicle stem cells
Capturing and profiling adult hair follicle stem cells
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DOI：
10.1038/nbt950
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发表时间：
2004-04-01
影响因子：
46.9
通讯作者：
Cotsarelis, G
中科院分区：
文献类型：
--
作者：
Morris, RJ;Liu, YP;Cotsarelis, G
毛囊隆突具有假定的上皮干细胞。这些细胞的表征由于不能在遗传上靶向隆突细胞而受到阻碍。在这里，我们使用一个角蛋白1 -15（Krt 1 -15，也称为K15）启动子与诱导型Cre重组酶构建体或与编码增强型绿色荧光蛋白（EGFP）的基因，这允许谱系分析和分离的细胞，以靶向小鼠隆突细胞。我们发现，成年小鼠的隆突细胞在正常毛囊周期中产生完整毛囊和毛发内的所有上皮细胞类型。分离后，成人Krt 1 -15-EGFP阳性细胞重建皮肤上皮的所有组成部分，并具有较高的增殖潜力比Krt 1 -15-EGFP阴性细胞。毛囊干细胞的遗传分析揭示了几个已知和未知的受体和信号通路对维持干细胞表型很重要。最终，这些发现为治疗脱发和其他皮肤和头发疾病提供了潜在的靶点。
The hair follicle bulge possesses putative epithelial stem cells. Characterization of these cells has been hampered by the inability to target bulge cells genetically. Here, we use a Keratin1-15 (Krt1-15, also known as K15) promoter to target mouse bulge cells with an inducible Cre recombinase construct or with the gene encoding enhanced green fluorescent protein (EGFP), which allow for lineage analysis and for isolation of the cells. We show that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling. After isolation, adult Krt1-15-EGFP-positive cells reconstituted all components of the cutaneous epithelium and had a higher proliferative potential than Krt1-15-EGFP-negative cells. Genetic profiling of hair follicle stem cells revealed several known and unknown receptors and signaling pathways important for maintaining the stem cell phenotype. Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair.