Preparation and evaluation of a novel bioactive glass/lysozyme/PLGA composite microsphere
Preparation and evaluation of a novel bioactive glass/lysozyme/PLGA composite microsphere
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DOI：
10.3109/03639045.2013.877485
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发表时间：
2015-03-01
影响因子：
3.4
通讯作者：
Xi, Jumei
中科院分区：
文献类型：
--
作者：
Liu, Hongfei;Shi, Shuangshuang;Xi, Jumei
目的：本研究的目的是制备一种掺有溶菌酶聚(D,L-丙交酯-乙交酯)(PLGA)的新型纳米生物陶瓷微球。方法：将纳米生物陶瓷作为可生物降解的缓释抗酸剂，以稳定溶菌酶在药物释放过程中的稳定性。首先采用溶胶-凝胶法制备纳米生物陶瓷，然后通过能量色散X射线分析、动态光散射和体外降解试验对其进行表征。其次，采用S/W/O/W乳液溶剂蒸发法制备了掺入纳米生物陶瓷的溶菌酶PLGA微球。通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和紫外圆二色性（UV CD）对微球进行表征。最后进行体外药物释放和生物活性测试。结果：纳米生物陶瓷的组成为58%SiO2、36%CaO、6%P2O5，平均粒径为295 nm。采用多重乳液法制备了掺有溶菌酶PLGA的纳米生物陶瓷微球。 SEM结果表明生物陶瓷均匀分布在PLGA微球中。体外溶菌酶释放试验结果显示，释放时间延长1个月。 FTIR和UVCD结果表明，药物释放过程中的溶菌酶具有与天然溶菌酶相似的二级结构构象。溶壁微球菌测试表明，掺入生物陶瓷的微球在 PLGA 降解导致的酸性环境中提供了长期的蛋白质稳定性，并且在 1 个月内释放的溶菌酶中 90% 以上以生物活性形式保存。结论：制备了一种新型掺入溶菌酶 PLGA 的生物陶瓷微球，具有持续蛋白质释放制剂的潜力。
Objective: The objective of this study was to fabricate a novel nano-bioceramics incorporated lysozyme poly (D, L-lactide-co-glycolide) (PLGA) microsphere.Methods: The nano-bioceramics was used as a biodegradable and sustained-release antacid to stabilize the lysozyme in the drug release process. First, the nano-bioceramics were prepared by sol-gel method, and then were characterized by energy dispersive X-ray analysis, dynamic light scattering and in vitro degradation test. Second, the lysozyme PLGA microsphere incorporated with nano-bioceramic was fabricated by the S/W/O/W emulsion solvent evaporation method. The microsphere was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and UV circular dichroism (UV CD). Finally the in vitro drug release and bioactivity test was carried out.Results: The composition of the nano-bioceramics was 58% SiO2, 36% CaO, 6% P2O5, and the average particle size was 295 nm. The nano-bioceramics incorporated lysozyme PLGA microspheres were prepared by the multi-emulsion method. The SEM results showed that the bioceramics was uniformly distributed in the PLGA microsphere. Results from in vitro lysozyme release test exhibited a prolonged release time for 1month. The FTIR and UVCD results suggested that the lysozyme in the drug release process had a similar secondary structure conformation to the native one. The Micrococcus lysodeikticus test showed that the microspheres incorporated with bioceramics provided long-term protein stability against the acidic environment resulted from PLGA's degradates and more than 90% of the lysozyme released over the 1 month period was preserved in a bioactive form.Conclusion: A novel bioceramics incorporated lysozyme PLGA microsphere was prepared with potentials for sustained protein release formulation.