Generation of Elf5-Cre knockin mouse strain for trophoblast-specific gene manipulation
Generation of Elf5-Cre knockin mouse strain for trophoblast-specific gene manipulation
复制标题
用于滋养层特异性基因操作的 Elf5-Cre 敲入小鼠品系的生成
DOI：
10.1002/dvg.23101
复制
发表时间：
2018-04-01
期刊：
影响因子：
1.5
通讯作者：
Lu, Jinhua
中科院分区：
文献类型：
--
作者：
Kong, Shuangbo;Liang, Guixian;Lu, Jinhua
Placental development is a complex and highly controlled process during which trophoblast stem cells differentiate to various trophoblast subtypes. The early embryonic death of systemic gene knockout models hampers the investigation of these genes that might play important roles during placentation. A trophoblast specific Cre mouse model would be of great help for dissecting out the potential roles of these genes during placental development. For this purpose, we generate a transgenic mouse with the Cre recombinase inserted into the endogenous locus of Elf5 gene that is expressed specifically in placental trophoblast cells. To analyze the specificity and efficiency of Cre recombinase activity in Elf5-Cre mice, we mated Elf5-Cre mice with Rosa26(mT/mG) reporter mice, and found that Elf5-Cre transgene is expressed specifically in the trophoectoderm as early as embryonic day 4.5 (E4.5). By E12.5, the activity of Elf5-Cre transgene was detected exclusively in all derivatives of trophoblast lineages, including spongiotrophoblast, giant cells, and labyrinth trophoblasts. In addition, Elf5-Cre transgene was also active during spermatogenesis, from spermatids to mature sperms, which is consistent with the endogenous Elf5 expression in testis. Collectively, our results provide a unique tool to delete specific genes selectively and efficiently in trophoblast lineage during placentation.
