OLIGONUCLEOTIDE PRIMERS FOR POLYMERASE CHAIN-REACTION AMPLIFICATION OF HUMAN-IMMUNOGLOBULIN VARIABLE GENES AND DESIGN OF FAMILY-SPECIFIC OLIGONUCLEOTIDE PROBES
OLIGONUCLEOTIDE PRIMERS FOR POLYMERASE CHAIN-REACTION AMPLIFICATION OF HUMAN-IMMUNOGLOBULIN VARIABLE GENES AND DESIGN OF FAMILY-SPECIFIC OLIGONUCLEOTIDE PROBES
复制标题
DOI：
10.1002/eji.1830210419
复制
发表时间：
1991-04-01
影响因子：
5.4
通讯作者：
WINTER, G
中科院分区：
文献类型：
--
作者：
MARKS, JD;TRISTEM, M;WINTER, G
在最近的工作中，聚合酶链反应（PCR）已被用于扩增重排的小鼠和人免疫球蛋白重链和κ轻链可变（V）基因。 我们优化了人V基因的PCR引物设计，并利用它们从人外周血淋巴细胞中扩增cDNA。 克隆和测序揭示了V基因的多样性，以及每个人V基因家族成员的存在。 序列比对后，我们确定了V基因家族内保守但家族间不同的区域，并利用该区域设计家族特异性寡核苷酸探针。
In recent work, the polymerase chain reaction (PCR) has been used to amplify rearranged mouse and human immunoglobulin heavy and kappa light chain variable (V) genes. Here we have optimized the design of the PCR primers for human V genes and used them to amplify cDNA from human peripheral blood lymphocytes. Cloning and sequencing revealed a diverse repertoire of V genes, and the presence of members of each human V gene family. After alignment of the sequences, we identified a region conserved within V gene families, but differing between families, and used this to design family-specific oligonucleotide probes.