Detection of abscisic acid (ABA) and expression profiles of ABA-related genes in a desert moss Syntrichia caninervis
Detection of abscisic acid (ABA) and expression profiles of ABA-related genes in a desert moss Syntrichia caninervis
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沙漠苔藓 Syntrichia caninervis 中脱落酸 (ABA) 的检测及 ABA 相关基因的表达谱
DOI：
10.1080/03736687.2021.1989927
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发表时间：
2021
影响因子：
1.9
通讯作者：
Daoyuan Zhang
中科院分区：
文献类型：
--
作者：
Yigong Zhang;Ayibaiheremu Mutailifu;Yi Zhang;Honglan Yang;Daoyuan Zhang
脱落酸（阿坝）是一种植物激素，在许多物种中具有调节环境胁迫反应的作用。内源性阿坝已被检测到在只有有限的数量的brathte species.MethodsSamples的干燥耐受mossSyntrichia caninervis Mitt。采用超高效液相色谱-质谱联用技术测定了银杏配子体内源阿坝含量。采用荧光定量PCR技术研究了ABA相关基因（ZEP、NCED 3、SnRK 2、ABI 1和ABI 5）在脱水、复水过程中以及对外源阿坝和低温胁迫的响应。 ABA相关基因的表达在非生物胁迫下发生变化。结论狗合毛藻能够产生阿坝，阿坝在该物种的胁迫响应中起着重要作用。本研究结果为进一步研究阿坝在犬刺链霉菌中的生理作用奠定了基础，并有助于加深对阿坝在犬刺链霉菌中的生物合成、代谢和信号转导途径的理解。
IntroductionAbscisic acid (ABA) is a plant hormone known to regulate environmental stress responses in various species. Endogenous ABA has been detected in only a limited number of bryophyte species.MethodsSamples of the desiccation-tolerant mossSyntrichia caninervisMitt. were analysed by ultra-performance liquid chromatography–mass spectrometry to determine the endogenous ABA content of the gametophores. Quantitative real-time PCR was conducted to investigate the transcript abundance of ABA-related genes (ZEP,NCED3,SnRK2,ABI1andABI5) during dehydration and rehydration and in response to exogenous ABA and cold stress.Key resultsOver a 24 h period of dehydration, the mean ABA concentration of the gametophores ranged from 7.96 to 30.87 ng/g dry weight, being maximal at 6 h. The expression of ABA-related genes changed in response to abiotic stress.ConclusionsThe results show thatSyntrichia caninerviscan generate ABA and suggest that ABA has crucial functions in the stress responses of this species. Our findings establish a foundation for further research on the physiological roles of ABA inS. caninervisand help extend our understanding of the biosynthetic, metabolic and signalling pathways of ABA in bryophytes.