Dedicated Hippocampal Inhibitory Networks for Locomotion and Immobility
Dedicated Hippocampal Inhibitory Networks for Locomotion and Immobility
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DOI：
10.1523/jneurosci.1076-17.2017
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发表时间：
2017-09-20
影响因子：
5.3
通讯作者：
Han, Edward B.
中科院分区：
文献类型：
--
作者：
Arriaga, Moises;Han, Edward B.
网络活动与动物运动密切相关;然而，在运动或不动期间选择性参与的海马回路仍然缺乏特征。在这里，我们研究了不同的运动状态是否编码差异遗传定义类海马中间神经元。为了表征中间神经元活动与运动之间的关系，我们在雄性和雌性小鼠的CA 1中使用了体内双光子钙成像，因为动物执行了虚拟现实（VR）跟踪运行任务。我们发现大多数生长抑素表达和小白蛋白表达的中间神经元的活动与运动呈正相关。令人惊讶的是，近五分之一的生长抑素或七分之一的小白蛋白中间神经元在运动过程中被抑制，在不动期间被激活。在解剖学上，生长抑素不动激活神经元的胞体比运动激活神经元的胞体小。此外，不动性激活的中间神经元分布在细胞层，生长抑素表达细胞主要在东方层和小清蛋白表达细胞主要在中层。重要的是，每个细胞的活动和运动之间的相关性随着时间的推移和VR环境的变化都是稳定的。我们的研究结果表明，海马神经元间的微电路是优先活跃在运动或不动期间。这些抑制性网络可以调节海马内“标记线”中的信息流，以在不同的行为状态下处理信息。
Network activity is strongly tied to animal movement; however, hippocampal circuits selectively engaged during locomotion or immobility remain poorly characterized. Here we examined whether distinct locomotor states are encoded differentially in genetically defined classes of hippocampal interneurons. To characterize the relationship between interneuron activity and movement, we used in vivo, two-photon calcium imaging in CA1 of male and female mice, as animals performed a virtual-reality (VR) track running task. We found that activity in most somatostatin-expressing and parvalbumin-expressing interneurons positively correlated with locomotion. Surprisingly, nearly one in five somatostatin or one in seven parvalbumin interneurons were inhibited during locomotion and activated during periods of immobility. Anatomically, the somata of somatostatin immobility-activated neurons were smaller than those of movement-activated neurons. Furthermore, immobility-activated interneurons were distributed across cell layers, with somatostatin-expressing cells predominantly in stratum oriens and parvalbumin-expressing cells mostly in stratum pyramidale. Importantly, each cell's correlation between activity and movement was stable both over time and across VR environments. Our findings suggest that hippocampal interneuronal microcircuits are preferentially active during either movement or immobility periods. These inhibitory networks may regulate information flow in "labeled lines" within the hippocampus to process information during distinct behavioral states.