Loss of E-cadherin and Acquisition of Vimentin in Epithelial-Mesenchymal Transition are Noble Indicators of Uterine Cervix Cancer Progression.
Loss of E-cadherin and Acquisition of Vimentin in Epithelial-Mesenchymal Transition are Noble Indicators of Uterine Cervix Cancer Progression.
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DOI：
10.4132/koreanjpathol.2012.46.4.341
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发表时间：
2012-08
影响因子：
--
通讯作者：
Myong NH
中科院分区：
文献类型：
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作者：
Myong NH
上皮-间质转化（EMT）在原位癌（CIS）病变的间质浸润中起关键作用。E-cadherin的缺失和vimentin的获得是EMT的两个关键步骤，它们是由与表皮生长因子受体（EGFR）过表达相关的Snail-1上调诱导的。然而，emt相关蛋白在人宫颈组织中的作用尚未完全阐明。在这项研究中，我们研究了emt相关蛋白在CIS，微创鳞状细胞癌（SCC）和侵袭性SCC中的免疫表达，以证明它们在肿瘤进展中的关键作用。在石蜡包埋组织芯片块上对81例CIS、17例微创和21例侵袭性SCC进行了Snail-1、EGFR、E-cadherin和vimentin的一抗免疫染色。EGFR和Snail-1蛋白均高表达，但三组间差异不显著。然而，在微创和侵袭性SCC病例中，E-cadherin的丢失和vimentin的获得明显高于CIS。E-cadherin和vimentin是评价CIS间质浸润的两个有用的EMT指标。然而，没有证据表明Snail-1和EGFR蛋白在宫颈癌的进展中发挥任何关键作用。
Epithelial-mesenchymal transition (EMT) has been known to play a key role in the stromal invasion of carcinoma in situ (CIS) lesion. Loss of E-cadherin and acquisition of vimentin are two critical steps in EMT, that are induced by Snail-1 upregulation associated with overexpression of epidermal growth factor receptor (EGFR). However, roles of EMT-related proteins in human cervical tissues have not been fully elucidated. In this study, we investigated the immunoexpressions of EMT-related proteins in CIS, microinvasive squamous cell carcinoma (SCC), and invasive SCC to demonstrate their key roles in tumor progression. Eighty one CIS, 17 microinvasive, and 21 invasive SCC cases were immunostained with primary antibodies for Snail-1, EGFR, E-cadherin, and vimentin on paraffin-embedded tissue microarray blocks. EGFR and Snail-1 proteins were highly expressed but the levels were not significantly different between the three groups. However, loss of E-cadherin and acquisition of vimentin were proven to occur significantly higher in microinvasive and invasive SCC cases than in CIS. E-cadherin and vimentin were found to be two useful indicators of EMT in evaluating stromal invasion of CIS. However, it was not demonstrated for Snail-1 and EGFR proteins to play any key role in the progression of cervix cancer.