The role of the liver in the production of free radicals during halothane anaesthesia in the rat. Quantification of N-tert-butyl-alpha-(4- nitrophenyl)nitrone (PBN)-trapped adducts in bile from halothane as compared with carbon tetrachloride.
The role of the liver in the production of free radicals during halothane anaesthesia in the rat. Quantification of N-tert-butyl-alpha-(4- nitrophenyl)nitrone (PBN)-trapped adducts in bile from halothane as compared with carbon tetrachloride.
复制标题
大鼠氟烷麻醉期间肝脏在自由基产生中的作用。
作者：
H. Hughes;I. M. George;J. Evans;C. Rowlands;G. Powell;C. Curtis
将氟烷或四氯化碳与自旋捕集器N-叔丁基-α-（4-硝基苯基）硝酮（PBN）共同给予装有胆管插管的大鼠或离体灌注肝脏制备物。氟烷麻醉下的大鼠产生大量的自由基，1小时内胆汁中排出5-9 nmol。在尿液或血浆中未检测到加合物。这些自由基的肝脏来源证实了对离体灌注肝脏的研究，其中氟烷加入到灌注液中导致相同PBN捕获的自由基加合物的胆汁消除。同样，给予CCl 4后，在整个动物和灌注肝脏制备物的胆汁中消除了相同的自由基物质。在灌注的肝脏中，3小时内，氟烷或CCl 4（以等摩尔浓度给药）产生的自由基的总胆汁消除大致相同（5-7 nmol）;然而，氟烷产生的自由基的消除在前1小时内更快。
Halothane or CCl4 was co-administered with the spin trap N-tert-butyl-alpha-(4-nitrophenyl)nitrone (PBN) to rats fitted with bile duct cannuli or to isolated perfused liver preparations. Rats maintained under halothane anaesthesia generated significant amounts of free radicals, and 5-9 nmol was excreted in bile over 1 h. No adducts were detected in urine or plasma. The hepatic origin of these free radicals was confirmed by studies on isolated perfused livers where the addition of halothane to the perfusate resulted in the biliary elimination of the same PBN-trapped radical adducts. Similarly, following CCl4 administration, the same radical species were eliminated in bile in the whole animal and the perfused liver preparation. In the perfused liver, over 3 h the total biliary elimination of radicals derived from halothane or CCl4 (administered at equimolar concentrations) was approximately the same (5-7 nmol); however, the elimination of halothane-derived radicals was more rapid over the first 1 h.