Profiling kidney microRNAs from juvenile grass carp (Ctenopharyngodon idella) after 56 days of oral exposure to decabromodiphenyl ethane
Profiling kidney microRNAs from juvenile grass carp (Ctenopharyngodon idella) after 56 days of oral exposure to decabromodiphenyl ethane
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DOI：
10.1016/j.jes.2015.09.022
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发表时间：
2016-06-01
影响因子：
6.9
通讯作者：
Hu, Guocheng
中科院分区：
文献类型：
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作者：
Gan, Lian;Xiong, Yuanyan;Hu, Guocheng
草鱼（Ctenopharyngodon idella）是我国重要的鱼类之一。十溴二苯乙烷（DBDPE）是一种工业上广泛使用的溴化阻燃剂，在中国南方的鱼类组织中有累积现象。有证据表明，十溴二苯乙烷对水生动物有毒，但其分子反应尚不清楚。microRNA（miRNAs）是一类长度为20-24个核苷酸的非编码负调控RNA，参与多种生物学过程。我们利用深度测序技术，准确而全面地分析了草鱼经口暴露于DBDPE 8周后肾脏miRNA的表达。在将测序数据与草鱼基因组和表达序列标签（EST）进行映射后，我们在测序的草鱼样品中鉴定了493个miRNAs，其中包括51个新的miRNAs。结果表明，在DBDPE暴露后，5种miRNAs显著下调，36种miRNAs显著上调（FDR < 0.001，1.5倍变化）。对两个样本中的4个miRNAs进行实时定量PCR（RT-qPCR），测序结果与RT-qPCR结果一致。本研究首次对草鱼miRNAs进行了全面鉴定，并首次对草鱼miRNAs在DBDPE暴露后的表达进行了分析。结果表明miRNA具有用作生物标志物的潜力。(C)2016中国科学院生态环境科学研究中心。由爱思唯尔公司出版
Grass carp (Ctenopharyngodon idella) is one of the most important species in China. Decabromodiphenyl ethane (DBDPE) is a brominated flame retardant that has been used widely in industry, and has been observed to accumulate in the tissues of fish from South China. Evidence has shown that DBDPE is toxic to aquatic animals, but the molecular response has been unclear. MicroRNAs (miRNAs) are small noncoding and negative regulatory RNAs that are 20-24 nucleotides in length, which are involved in a wide range of biological processes. We took advantage of deep-sequencing techniques to accurately and comprehensively profile the kidney miRNA expression of grass carp after 8 weeks of oral exposure to DBDPE. After mapping sequencing data to the genome and Expressed Sequence Tags (ESTs) of grass carp, we identified 493 miRNAs in the sequenced grass carp samples, which included 51 new miRNAs. The results indicated that 5 miRNAs were significantly down-regulated and 36 miRNAs were significantly up-regulated (FDR < 0.001, 1.5-fold change) after DBDPE exposure. Real-time quantitative PCR (RT-qPCR) was performed on 4 miRNAs from the two samples, and the sequencing and RT-qPCR data were consistent. This study provides the first comprehensive identification of grass carp miRNAs, and the first expression analysis of grass carp miRNAs following DBDPE exposure. The results indicated that miRNAs have potential for use as biomarkers. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.