Losartan prevents bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder
Losartan prevents bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder
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DOI：
10.1002/nau.24567
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发表时间：
2021-01-01
影响因子：
2
通讯作者：
Bauer, Stuart B.
中科院分区：
文献类型：
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作者：
He, Yu L.;Wen, Jian G.;Bauer, Stuart B.
目的探讨氯沙坦预防神经源性瘫痪大鼠(NPB)膀胱纤维化和保护肾功能的作用。材料与方法将大鼠分为切断脊神经组(TSNG)、切断脊神经+氯沙坦组(LSTG)和对照组(CG)。术后第32天，用膀胱测压仪测定TSNG组和LSTG组的膀胱容量(BC)、膀胱顺应性(Delta C)、膀胱漏出压(P-ves.leak)，以及CG组的BC、Delta C和膀胱阈值压力(P-ves.thre)。检测肾功能和血中血管紧张素Ⅱ(Ang II)的表达，并检测膀胱组织中Ang II、Ang II 1型受体(AT1)、转化生长因子β1(TGFβ1)、胶原和胶原纤维蛋白的含量。TSNG组和LSTG组的P-ves.lear显著高于CG组的P-ves.Thre。TSNG组和LSTG组肾功能均较对照组明显降低，但LSTG组肾功能优于TSNG组。TSNG组和LSTG组大鼠血液和膀胱组织中Ang II含量明显高于对照组。AT1在所有大鼠的膀胱组织中均有表达。结论氯沙坦可能通过阻断血管紧张素Ⅱ/血管紧张素Ⅱ/血管内皮细胞生长因子1/转化生长因子β1上调的信号转导途径来预防肾小球纤维化，从而减轻肾损害。
Aims To investigate the effect of losartan on preventing bladder fibrosis and protecting renal function in rats with neurogenic paralysis bladder (NPB).Materials and Methods Rats were assigned to the transecting spinal nerves group (TSNG), transecting spinal nerves + losartan group (LSTG), and control group (CG). On Day 32 postsurgery, bladder capacity (BC), bladder compliance (Delta C), bladder leakage pressure (P-ves.leak) of TSNG and LSTG while BC, Delta C, and bladder threshold pressure (P-ves.thre) of CG were measured by cystometry in each cohort. Renal function and the expression quantity of Angiotensin II (Ang II) in blood were detected, in addition Ang II, Ang II Type 1 receptor (AT1), transformation growth factor beta 1 (TGF beta 1), Collagen, and collagen fibrin in the bladder tissue were detected too.Results Delta C in TSNG and LSTG decreased significantly compared to the CG. P-ves.leak in TSNG and LSTG were significantly higher than P-ves.thre in CG. Renal function of both TSNG and LSTG decreased significantly compared with the CG, but renal function in LSTG was better than in TSNG. Ang II in blood and bladder tissue in TSNG and LSTG increased significantly compared with CG. AT1 was expressed in the bladder tissue of all rats. The TGF beta 1, Collagen, and collagen fibrin expression level increased significantly in TSNG compared with LSTG and CG, while these levels were not significantly different between CG and LSTG.Conclusion Losartan might prevent NPB fibrosis by stopping the upregulated signaling of Ang II/AT1/TGF beta 1 and consequently may reduce kidney damage from occurring.