AXONS - ISOLATION FROM MAMMALIAN CENTRAL NERVOUS-SYSTEM
AXONS - ISOLATION FROM MAMMALIAN CENTRAL NERVOUS-SYSTEM
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DOI：
10.1126/science.175.4028.1370
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发表时间：
1972-01-01
期刊：
影响因子：
56.9
通讯作者：
RAINE, CS
中科院分区：
文献类型：
--
作者：
DEVRIES, GH;NORTON, WT;RAINE, CS
在含盐的缓冲蔗糖溶液中离心白质匀浆，产生有髓鞘轴突的漂浮层。当它们悬浮在低渗缓冲液中时，髓磷脂膨胀并从轴突上剥离。然后通过离心将轴突与髓磷脂分离。由此产生的制剂由长度可达 200 微米、直径在 0.3 至 5.0 微米之间的可变过程组成。轴突含有神经丝和线粒体，但没有明显的轴膜或神经管。该制剂含有脑苷脂和脑硫脂，但基本上不含髓磷脂。
Centrifugation of a homogenate of white matter, in a solution of buffered sucrose containing salt, produces a floating layer of myelinated axons. When these are suspended in hypotonic buffer, the myelin swells and strips away from the axon. Axons are then separated from the myelin by centrifugation. The resulting preparation consists of a variable population of processes with lengths up to 200 micrometers and diameters between 0.3 and 5.0 micrometers. The axons contain neurofilaments and mitochondria, although no axolemma or neurotubules are evident. The preparation contains cerebroside and sulfatide, yet is essentially free of myelin.