Limited variation in the major urinary proteins of laboratory mice
Limited variation in the major urinary proteins of laboratory mice
复制标题
DOI：
10.1016/j.physbeh.2008.10.005
复制
发表时间：
2009-02-16
影响因子：
2.9
通讯作者：
Hurst, Jane L.
中科院分区：
文献类型：
--
作者：
Cheetham, Sarah A.;Smith, Abigail L.;Hurst, Jane L.
一组特殊的气味通讯蛋白(主要尿蛋白(MUP))的个体变异提供了一个遗传身份签名，该签名奠定了个体和亲属识别以及野生家鼠杂合性评估的基础。在这里，我们研究了来自三个主要血统(Castle，C57，Swiss)的30个经典品系的实验室小鼠的MUP变异保留的程度。在经典实验室菌株的早期阶段，尿MUP模式的正常野生型变异似乎已经丢失。来自城堡和瑞士血统的所有菌株都有相同的“个人”MUP模式，与极少数创始人的共同祖先一致，而来自C57血统的菌株则有不同的模式。值得注意的是，在瑞士近交系ICR(CD-1)品系中，MUP模式的个体差异并不比自交系的典型品系更大。尽管MUP表达的数量变化的功能意义尚未确定，但雄鼠的MUP表达水平是雄鼠的2-8倍，而大多数雄性野鼠表达的MUP在C57雄鼠中表达，但在Castle和Swiss雄鼠中表达不同，在某些品系中雌性野鼠表达的MUP有时也不同。品系内和品系之间的MUP模式缺乏正常变异，这对实验室小鼠在行为或神经生理学研究中的使用具有重要意义，该研究旨在调查社会认知或配偶选择。(C)2008 Elsevier Inc.保留所有权利。
Individual variation in a specialised set of scent communication proteins, the major urinary proteins (MUPs), provides a genetic identity signature that underlies individual and kin recognition, and the assessment of heterozygosity in wild house mice. Here we examine the extent to which MUP variation is retained among 30 classical strains of laboratory mice from three main lineages (Castle, C57, Swiss). Normal wild-type variation in urinary MUP pattern appears to have been lost at an early stage in the derivation of the classical laboratory strains. All strains from the Castle and Swiss lineages shared the same "individual" MUP pattern, consistent with common ancestry from very few founders, while those from the C57 lineage shared a different pattern. Notably, individual variation in MUP pattern was no greater within the Swiss outbred ICR (CD-1) strain than typical for inbred strains. Total urinary protein concentration varied considerably between even closely related substrains, together with minor variation in the relative amount of each MUP isoform expressed, although the functional significance of such quantitative variation in MUP expression has yet to be established, Expression was 2-8 fold higher among males, while a MUP expressed by most male but not female wild mice was expressed by C57 males but variably among Castle and Swiss males and occasionally by females in some strains. The lack of normal variation in MUP patterns within and between strains has important implications for the use of laboratory mice in behavioural or neurophysiological research investigating social recognition or mate choice. (C) 2008 Elsevier Inc. All rights reserved.