Referencing laser and ultrasonic height measurements of barley cultivars by using a herbometre as standard
Referencing laser and ultrasonic height measurements of barley cultivars by using a herbometre as standard
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DOI：
10.1071/cp16238
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发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Schmidhalter, Urs
中科院分区：
文献类型：
--
作者：
Barmeier, Gero;Mistele, Bodo;Schmidhalter, Urs
植株高度的评估是农艺学和育种决策的重要因素;然而，目前的田间表型分析，例如视觉评分或使用标尺，是耗时的、劳动密集型的、昂贵的和主观的。对于农学家和植物育种家来说，最常用的测量植物高度的方法仍然是米棒。在一项为期3年的研究中，我们采用了一个herbometre类似的上升板米作为参考方法，以获得加权株高的大麦品种，并评估车载超声波和激光距离传感器。2013年、2014年和2015年分别对30个春大麦品种和14个和60个冬大麦品种进行了试验。该herbometer是非常适合作为一个参考方法，允许增加面积，并易于处理。小区内的测草计测量值显示出非常低的变异系数。在2013年、2014年和2015年分别观察到测草仪和超声波距离传感器测量值之间的良好和密切关系（R-2 = 0.59、0.76、0.80），这也表明遗传力值增加。因此，这两个传感器能够区分大麦品种在标准育种试验。对于传感器，我们观察到与herbometre测量相比，操作时间快4倍，测量点增加6倍。基于这些结果，我们得出结论，距离传感器代表了一个强大的和经济的高通量表型工具，育种家和植物科学家估计株高和区分品种的农艺决策和育种活动可能也适用于其他小粒谷物与密集的作物立场。特别地，超声波距离传感器可以反映农艺学和生理学相关的植物高度信息。
Assessment of plant height is an important factor for agronomic and breeder decisions; however, current field phenotyping, such as visual scoring or using a ruler, is time consuming, labour intensive, costly and subjective. For agronomists and plant breeders, the most common method used to measure plant height is still a meter stick. In a 3-year study, we have adopted a herbometre similar to a rising plate meter as a reference method to obtain the weighted plant height of barley cultivars and to evaluate vehicle-based ultrasonic and laser distance sensors. Sets of 30 spring barley cultivars and 14 and 60 winter barley cultivars were tested in 2013, 2014 and 2015, respectively. The herbometre was well suited as a reference method allowing for an increased area and was easy to handle. The herbometre measurements within a plot showed very low coefficients of variation. Good and close relationships (R-2 = 0.59, 0.76, 0.80) between the herbometre and the ultrasonic distance sensor measurements were observed in the years 2013, 2014 and 2015, respectively, demonstrating also increased values of heritability. Hence, both sensors were able to differentiate among barley cultivars in standard breeding trials. For the sensors, we observed a 4-fold faster operating time and 6-fold increase of measurement points compared with the herbometre measurement. Based on these results, we conclude that distance sensors represent a powerful and economical high-throughput phenotyping tool for breeders and plant scientists to estimate plant height and to differentiate cultivars for agronomic decisions and breeding activities potentially being also applicable in other small grain cereals with dense crop stands. Particularly, ultrasonic distance sensors may reflect an agronomically and physiologically relevant plant height information.