DETECTION OF CHARGED PROTEINS BY MEANS OF IMPEDANCE MEASUREMENTS
DETECTION OF CHARGED PROTEINS BY MEANS OF IMPEDANCE MEASUREMENTS
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DOI：
10.1016/0925-4005(92)80039-z
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发表时间：
1992-01-01
影响因子：
8.4
通讯作者：
REINHOUDT, DN
中科院分区：
文献类型：
--
作者：
KRUISE, J;RISPENS, JG;REINHOUDT, DN
含有带电蛋白质的膜的电阻是膜中存在的电解质离子的浓度和迁移率的函数，因此它取决于唐南效应。我们能够通过简单的交流阻抗法测定沉积在ISFET上的这种膜的电阻。阻抗测量作为实例，使用含有交联溶菌酶的膜。我们发现膜电阻是成反比的固定电荷密度，在良好的协议与基于Donnan理论的理论描述。
The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory.