Effects of heating on the interaction of lipid and zein in a dry powder system
Effects of heating on the interaction of lipid and zein in a dry powder system
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DOI：
10.1021/jf030677g
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发表时间：
2004-06-02
影响因子：
6.1
通讯作者：
Mori, T
中科院分区：
文献类型：
--
作者：
Mizutani, Y;Matsumura, Y;Mori, T
研究了热处理对干粉体系中脂质与玉米醇溶蛋白相互作用的影响。将亚麻酸乙酯（LAE）与玉米醇溶蛋白粉混合。差示扫描量热法显示干粉玉米醇溶蛋白的玻璃化转变温度（T - g）约为107℃。玉米醇溶蛋白 - LAE混合粉的热谱图在玉米醇溶蛋白的T - g附近显示出放热转变。利用傅里叶变换红外光谱（FT - IR）检测热处理（即温度从25℃升高到160℃）对玉米醇溶蛋白结构的改变。对含和不含LAE的粉状玉米醇溶蛋白进行热处理，导致α - 螺旋、β - 转角和β - 折叠增加，同时分子间氢键结合的β - 折叠和无规卷曲减少。对于含LAE的粉末，二级结构的这种变化更为剧烈。玉米醇溶蛋白 - LAE混合粉受热还导致FT - IR光谱中源于LAE的峰降低。这些结果表明，热处理诱导了粉状体系中玉米醇溶蛋白与LAE的相互作用。通过测量过氧化值研究了加热对干粉玉米醇溶蛋白抗氧化活性的影响。当玉米醇溶蛋白 - LAE混合粉在储存前受热时，LAE的氧化在7天内受到抑制，而在未进行热处理的情况下，LAE在1天内就被氧化。
The effects of heat treatment on the interaction of lipid and zein in a dry powder system were investigated. Linolenic acid ethyl ester (LAE) was mixed with the zein powder. The glass transition temperature (T-g) for the dry powder zein was shown to be similar to107 degreesC by differential scanning calorimetry. The thermogram of the zein-LAE mixed powder showed an exothermic transition near the T-g of zein. Fourier transform infrared (FT-IR) spectroscopy was used for detecting the structural changes in zein by heat treatment, that is, elevating the temperature from 25 to 160 degreesC. The heat treatment of the powdery zein with and without LAE caused increases in the alpha-helix, beta-turn, and beta-sheet, concomitant with decreases in the intermolecular hydrogen-bonded beta-sheet and random coil. Such changes in the secondary structure were more drastic for the powder with LAE, The heating of the zein-LAE mixed powder also caused decreases in the peaks originating from LAE in the FT-IR spectra. These results suggest that the heat treatment induced the interaction of the zein and LAE in the powdery system. The influence of heating on the antioxidative activity of dry powder zein was studied by measurements of the peroxide value. When zein-LAE mixed powder was heated before storage, the oxidation of LAE was inhibited for 7 days, whereas LAE was oxidized within 1 day in the absence of heat treatment.