Nematicidal Activities of 4-Quinolone Alkaloids Isolated from the Aerial Part of Triumfetta grandidens against Meloidogyne incognita
Nematicidal Activities of 4-Quinolone Alkaloids Isolated from the Aerial Part of Triumfetta grandidens against Meloidogyne incognita
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DOI：
10.1021/jf504572h
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发表时间：
2015-01-14
影响因子：
6.1
通讯作者：
Kim, Jin-Cheol
中科院分区：
文献类型：
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作者：
Jang, Ja Yeong;Quang Le Dang;Kim, Jin-Cheol
Triumfetta grandidens（椴科）地上部分的甲醇提取物对南方根结线虫具有高度活性，在 500 μg/mL 浓度下，暴露后 48 小时，第二阶段幼虫 (J2s) 死亡率为 100%。通过生物测定引导的分馏和仪器分析，分离并鉴定了两种 4-喹诺酮生物碱：新生物碱 waltherione E (1) 和 waltherione A (2)，并鉴定为杀线虫化合物。根据死亡率和对卵孵化的影响评估分离的化合物对南方根结线虫的杀线虫活性。化合物1和2对南方根结线虫J2表现出较高的死亡率，48小时时的EC50值分别为0.09和0.27μg/mL。化合物1和2对卵孵化也表现出相当大的抑制作用，在1.25μg/mL浓度下暴露7天后，分别抑制91.9%和87.4%的卵孵化。两种4-喹诺酮生物碱的生物活性与阿维菌素相当。此外，利用大红椿地上部分粗提物进行的盆栽实验表明，在1000μg/mL的浓度下，它可以完全抑制植物根部瘿的形成。这些结果表明，大线虫及其生物活性 4-喹诺酮生物碱可在有机农业中用作有效的植物杀线虫剂。
The methanol extract of the aerial part of Triumfetta grandidens (Tiliaceae) was highly active against Meloidogyne incognita, with second-stage juveniles (J2s) mortality of 100% at 500 mu g/mL at 48 h post-exposure. Two 4-quinolone alkaloids, waltherione E (1), a new alkaloid, and waltherione A (2), were isolated and identified as nematicidal compounds through bioassay-guided fractionation and instrumental analysis. The nematicidal activities of the isolated compounds against M. incognita were evaluated on the basis of mortality and effect on egg hatching. Compounds 1 and 2 exhibited high mortalities against J2s of M. incognita, with EC50 values of 0.09 and 0.27 mu g/mL at 48 h, respectively. Compounds 1 and 2 also exhibited a considerable inhibitory effect on egg hatching, which inhibited 91.9 and 87.4% of egg hatching, respectively, after 7 days of exposure at a concentration of 1.25 mu g/mL. The biological activities of the two 4-quinolone alkaloids were comparable to those of abamectin. In addition, pot experiments using the crude extract of the aerial part of T. grandidens showed that it completely suppressed the formation of gall on roots of plants at a concentration of 1000 mu g/mL. These results suggest that T. grandidens and its bioactive 4-quinolone alkaloids can be used as a potent botanical nematicide in organic agriculture.