MOLECULAR-CLONING OF A MURINE FIBRONECTIN RECEPTOR AND ITS EXPRESSION DURING INFLAMMATION - EXPRESSION OF VLA-5 IS INCREASED IN ACTIVATED PERITONEAL-MACROPHAGES IN A MANNER DISCORDANT FROM MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II
MOLECULAR-CLONING OF A MURINE FIBRONECTIN RECEPTOR AND ITS EXPRESSION DURING INFLAMMATION - EXPRESSION OF VLA-5 IS INCREASED IN ACTIVATED PERITONEAL-MACROPHAGES IN A MANNER DISCORDANT FROM MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II
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DOI：
10.1084/jem.169.5.1589
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发表时间：
1989-05-01
影响因子：
15.3
通讯作者：
BROWN, EJ
中科院分区：
文献类型：
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作者：
HOLERS, VM;RUFF, TG;BROWN, EJ
人纤维连接蛋白受体(VLA-5)。和测试版。用链状探针在硫代乙二醇酸诱导的腹膜渗出物细胞cDNA文库中鉴定它们的同源物。两个α的序列分析。和测试版。链相关的小鼠克隆揭示了.apprx。通过核苷酸和衍生的氨基酸序列比较，与人类同源物的同源性为90%。可检测到的阿尔法。链转录本主要存在于腹膜巨噬细胞的总RNA中。.测试版。然而，在肺、心、脑和肾中检测到较高水平的链表达，这表明存在一个与人类相似的大鼠VLA家族。比较静息的腹膜巨噬细胞和使用炎症刺激的巨噬细胞的表达水平的分析表明。以硫代乙醇酸盐或单核细胞增多性李斯特菌为刺激物诱导细胞，链信息和表面LVA-5的表达显著增加。有趣的是，测试版。链信息不受这些炎性刺激的影响，表明VLA-5的表达受VLA-5α的调节。链接消息级别。这些结果表明，巨噬细胞VLA-5的表达在体内是可以调节的，这可能是炎症灶中巨噬细胞与纤维连接蛋白结合或黏附的重要机制。
Human fibronectin receptor (VLA-5) .alpha. and .beta. chain probes were used to identify their mouse homologues in a thioglycollate-elicited peritoneal exudate cell cDNA library. Sequence analysis of both .alpha. and .beta. chain-related murine clones revealed .apprx. 90% homology to their human counterparts by both nucleotide and derived amino acid sequence comparisons. Detectable .alpha. chain transcripts were seen predominantly in total RNA of peritoneal macrophages. .beta. chain expression, however, was detected at higher levels in lung, heart, brain, and kidney, suggesting the presence of a large murine VLA family similar to the human family. Analysis of levels of expression comparing resting peritoneal macrophages with macrophages elicited using inflammatory stimuli indicated that .alpha. chain message and surface LVA-5 expression were significantly increased using thioglycollate or Listeria monocytogenes as stimuli to elicit cells. Interestingly, .beta. chain message was unaffected by these inflammatory stimuli, suggesting that VLA-5 expression is regulated by VLA-5 .alpha. chain message levels. These results indicate that macrophage VLA-5 expression can be modulated in vivo and may provide an important mechanism by which macrophages are recruited to or adhere to fibronectin in inflammatory foci.