Honokiol inhibits carotid artery atherosclerotic plaque formation by suppressing inflammation and oxidative stress
Honokiol inhibits carotid artery atherosclerotic plaque formation by suppressing inflammation and oxidative stress
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和厚朴酚通过抑制炎症和氧化应激来抑制颈动脉粥样硬化斑块的形成
DOI：
10.18632/aging.103120
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发表时间：
2020-05-15
期刊：
影响因子：
5.2
通讯作者：
Wu, An-Hua
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Cheng, Peng;Wu, An-Hua
和厚朴酚是从中草药厚朴中提取的天然活性化合物。在这项研究中，在正常饮食 (ND) 或西式饮食 (WD) 喂养十周的 ApoE-/- 小鼠模型中评估和厚朴酚在颈动脉粥样硬化病变发展中的作用。两周后，通过手术将血管周围项圈放置在小鼠的右侧颈总动脉上。然后，WD喂养的小鼠腹腔注射和厚朴酚（10或20 mg/kg）或灌胃给予10 mg/kg阿托伐他汀钙，每天一次，持续八周。之后，切除右侧颈总动脉以进行进一步实验。结果表明和厚朴酚显着抑制动脉粥样硬化病变的发展。此外，和厚朴酚还能下调促炎标志物的表达，如肿瘤坏死因子-α、白细胞介素 (IL)-6 和 IL-1 β。此外，和厚朴酚处理降低了活性氧水平并增强了超氧化物歧化酶活性。一氧化氮水平、诱导型一氧化氮合酶 (iNOS) 表达和核因子-κ B 通路的异常激活也受到和厚朴酚处理的显着抑制。总之，这些发现表明和厚朴酚可以防止颈动脉中动脉粥样硬化斑块的形成，并且可能是治疗颈动脉粥样硬化狭窄的有效候选药物。
Honokiol is a natural active compound extracted from Chinese herbal medicine, Magnolia officinalis. In this study, the role of honokiol in the development of carotid artery atherosclerotic lesions was evaluated in an ApoE-/- mouse model fed with a normal diet (ND) or a Western-type diet (WD) for ten weeks. After first two weeks, a perivascular collar was surgically placed on the right common carotid arteries of the mice. Then, WD-fed mice were intraperitoneally injected with honokiol (10 or 20 mg/kg) or administrated with 10 mg/kg atorvastatin calcium by gavage once a day for eight weeks. After that, the right common carotid arteries were excised for further experiments. The result showed that honokiol substantially inhibited the development of atherosclerotic lesions. Furthermore, honokiol downregulated the expression of pro-inflammatory markers, like tumor necrosis factor-alpha, interleukin (IL)-6, and IL-1 beta. Additionally, honokiol treatment decreased reactive oxygen species level and enhanced superoxide dismutase activity. Nitric oxide level, inducible nitric oxide synthase (iNOS) expression, and aberrant activation of nuclear factor-kappa B pathway were also significantly inhibited by honokiol treatment. Together, these findings suggest that honokiol protects against atherosclerotic plaque formation in carotid artery, and may be an effective drug candidate for the treatment of carotid artery atherosclerotic stenosis.