The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides
The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides
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DOI：
10.1016/j.bbrc.2010.10.059
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发表时间：
2010-11-26
影响因子：
3.1
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
--
作者：
Sakurai, Takanobu;Misaka, Takumi;Abe, Keiko
甜味和苦味分别是食物接受和拒绝的关键决定因素。蔗糖和果糖等糖类通常被认为是甜的，但并非所有的糖都是甜的，甚至异头体也可能具有完全不同的味道。例如，龙胆二糖是苦的，而其异麦芽糖的异头体是甜的。对异源表达人味觉受体的人胚肾293 T细胞进行成像分析，以证明龙胆二糖激活hTAS 2 R16（苦味受体）而非hT 1 R2/hT 1 R3（甜味受体）。相反，异麦芽糖激活hT 1 R2/hT 1 R3。吡喃葡萄糖苷如水杨苷共享一个共同的结合位点的hTAS 2 R16（C）2010爱思唯尔公司版权所有
Sweetness and bitterness are key determinants of food acceptance and rejection respectively Sugars such as sucrose and fructose are generally recognized as sweet However not all sugars are sweet and even anomers may have quite different tastes For example gentiobiose is bitter whereas its anomer isomaltose is sweet Despite this unique sensory character the molecular basis of the bitterness of gentiobiose remains to be clarified In this study we used calcium imaging analysis of human embryonic kidney 293T cells that heterologously expressed human taste receptors to demonstrate that gentiobiose activated hTAS2R16 a bitter taste receptor but not hT1R2/hT1R3 a sweet taste receptor In contrast isomaltose activated hT1R2/hT1R3 As a result these anomers elicit different taste sensations Mutational analysis of hTAS2R16 also indicated that gentiobiose and beta-D-glucopyranosides such as salicin share a common binding site of hTAS2R16 (C) 2010 Elsevier Inc All rights reserved