A 7.6 kb DNA region from Streptomyces kasugaensis M338-M1 includes some genes responsible kasugamycin biosynthesis
A 7.6 kb DNA region from Streptomyces kasugaensis M338-M1 includes some genes responsible kasugamycin biosynthesis
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DOI：
10.7164/antibiotics.51.341
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发表时间：
1998-03-01
影响因子：
3.3
通讯作者：
Hori, M
中科院分区：
文献类型：
--
作者：
Ikeno, S;Tsuji, T;Hori, M
从春日链霉菌（Streptomyces kasugaensis）M338-M1中分离到一个7.6kb的PstI-KpnI DNA片段，该片段含有与春雷霉素乙酰转移酶基因（kac）高度相似的序列。在该区域共识别出9个开放阅读框（ORF），命名为ORF A、B、C、D、E、F、G、H和I，但ORF A是不完整的。ORF G的运行方向与其他ORF相反。推导的氨基酸序列与S. kasugaensis MB 273-C4，另一种春雷霉素（KSM）生产者。转化大肠coliJM 109中的ORF为H，使菌株对KSM高度抗性。ORF A、C和D产物的氨基酸序列分别与E.大肠杆菌（26%）、恶臭假单胞菌（Pseudomonasputida）的β-丙氨酸：丙酮酸转氨酶（32%）和灰色链霉菌（Streptomycesgriseus）的dTDP-D-葡萄糖4，6-脱氢酶（StrE）（37%）。strE样ORF（ORF D）似乎是负责形成kasugamine部分的6-脱氧结构的基因。ORF A和ORF C也可能在KSM生物合成中起作用。两者合计，我们的分析强烈表明，该DNA区域包括至少一部分的基因簇的KSM生物合成。
A 7.6 kb PstI-KpnI DNA fragment including a sequence highly similar to kasugamycin acetyltransferase gene (kac) was isolated from Streptomyces kasugaensis M338-M1 and sequenced. Nine open reading frames (ORFs), designated as ORF A, B, C, D, E, F, G, H and I, were recognized in this region, although ORF A was incomplete. ORF G runs in the opposite direction to the others. The amino acid sequence deduced from ORF H showed 98% similarity to that of the kasugamycin acetyltransferase from S. kasugaensis MB273-C4, another kasugamycin (KSM) producer. Transformation of E. coli JM109 with ORF H made the strain highly resistant to KSM. The deduced amino acid sequences of the ORF A, C and D products were similar, respectively, to glucosyltransferase I from E. coli (26%), beta-alanine:pyruvate transaminase from Pseudomonas putida (32%) and dTDP-D-glucose 4,6-dehydratase (StrE) from Streptomyces griseus (37%). The strE-like ORF (ORF D) seems to be the gene responsible for formation of the 6-deoxy structure of the kasugamine moiety. ORF A and ORF C are also likely to have roles in KSM biosynthesis. Taken together, our analyses strongly suggest that this DNA region includes at least a part of the gene cluster of KSM biosynthesis.