Diagnosis of Nitrosative Stress by Quantitative EPR-Spectroscopy of Epidermal Cells
Diagnosis of Nitrosative Stress by Quantitative EPR-Spectroscopy of Epidermal Cells
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表皮细胞定量 EPR 光谱诊断亚硝化应激
DOI：
10.1007/s10517-013-2042-3
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发表时间：
2013
影响因子：
0.7
通讯作者：
A. N. Shchukov
中科院分区：
文献类型：
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作者：
V. Petukhov;L. Baumane;E. D. Reste;T. Zvagule;M. Romanova;N. I. Shushkevich;L. T. Sushkova;S. V. Skavronsky;A. N. Shchukov
用电子顺磁共振（EPR）法对45名切尔诺贝利核事故清理者、30名与辐射接触的放射性机构工作人员和50名弗拉基米尔州立大学学生的表皮衍生物（头发）中的一氧化氮（NO）和铁（Fe）进行了定量分析，其中大部分被诊断为缺铁，即缺铁性贫血（4人）和隐性缺铁（34人）。在Radiopan EPR光谱仪上进行测量。发现以二乙基二硫代氨基甲酸酯为捕获剂的定量EPR光谱法可成功地用于测定头发等生物基质中的NO自由基活性。结果发现，光谱图中NO-自由基信号的强度取决于Fe水平（不仅在被分析的底物中，而且在整个生物体中）。
Quantitative assay of nitric oxide (NO) and iron (Fe) was carried out by the electron paramagnetic resonance (EPR) method in epidermal derivative (hair) of 45 liquidators of the Chernobyl accident, 30 employees of radiological institutions having contact with radiation, and 50 students of Vladimir State University, most of which were diagnosed with iron deficiency, namely iron deficiency anemia (4 persons) and latent iron deficiency (34 persons). Measurements were performed on a Radiopan EPR-spectrometer. It was found that the method of quantitative EPR-spectroscopy using diethyldithiocarbamate as a trapin vitrocould be successfully used to measure NO-radical activity in such a biological substrate as hair. It was found that the intensity of NO-radical signal in the spectrogram depends on the Fe level (not only in the analyzed substrate, but also in the whole organism).