The mobility of chondroitin sulfate in articular and artificial cartilage characterized by 13C magic-angle spinning NMR spectroscopy.
The mobility of chondroitin sulfate in articular and artificial cartilage characterized by 13C magic-angle spinning NMR spectroscopy.
复制标题
通过 13C 魔角旋转核磁共振波谱表征硫酸软骨素在关节和人工软骨中的迁移率
DOI：
10.1002/bip.21386
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发表时间：
2010
期刊：
影响因子：
2.9
通讯作者：
Daniel Huster
中科院分区：
文献类型：
--
作者：
Holger A. Scheidt;Alvicler Magalhäes;Stephanie Schibur;Eduardo R. de Azevedo;Tito J. Bonagamba;Ovidiu Pascui;Ronny Schulz;Detlef Reichert;Daniel Huster
我们研究了关节软骨中的主要大分子之一--硫酸软骨素的分子动力学。应用~(13)C高分辨率魔角旋转核磁共振技术，可以抑制软骨中所有刚性大分子的核磁共振信号，使高流动性的硫酸软骨素得以专属检测。该技术还被用于检测人工组织工程软骨中的硫酸软骨素。这种组织工程材料的基础是在胶原凝胶中培养的产生基质的软骨细胞，它应该提供尽可能接近天然软骨的特性。测定两种组织中硫酸软骨素的核松弛时间。尽管组织工程软骨的T1松弛时间非常相似，但T2松弛时间明显较短。这表明硫酸软骨素在天然软骨和人工软骨中的运动是不同的。利用运动相关时间的广义分布函数，模拟了硫酸软骨素在天然和组织工程软骨中的核弛豫时间。虽然描述天然和人工软骨中硫酸软骨素的微观分子动力学需要运动关联时间的广义分布函数相同，但从模型中提取的运动关联时间的显著差异表明人工组织并不完全满足自然理想的标准。宏观生物力学弹性测量也可以证实这一点。然而，这些结果表明，核磁共振对于研究人工工程组织的质量是一个有用的工具。©2010 Wiley期刊，Inc.生物聚合物93：520-532,2010。本文最初作为接受的预印本在网上发布。“在线出版”日期与预印本相对应。您可以通过发送电子邮件给Biopolples编辑办公室来索要预印本的副本，电子邮件地址是biopolplers@wiley.com
We have studied the molecular dynamics of one of the major macromolecules in articular cartilage, chondroitin sulfate. Applying13C high‐resolution magic‐angle spinning NMR techniques, the NMR signals of all rigid macromolecules in cartilage can be suppressed, allowing the exclusive detection of the highly mobile chondroitin sulfate. The technique is also used to detect the chondroitin sulfate in artificial tissue‐engineered cartilage. The tissue‐engineered material that is based on matrix producing chondrocytes cultured in a collagen gel should provide properties as close as possible to those of the natural cartilage. Nuclear relaxation times of the chondroitin sulfate were determined for both tissues. Although T1relaxation times are rather similar, the T2relaxation in tissue‐engineered cartilage is significantly shorter. This suggests that the motions of chondroitin sulfate in natural and artificial cartilage are different. The nuclear relaxation times of chondroitin sulfate in natural and tissue‐engineered cartilage were modeled using a broad distribution function for the motional correlation times. Although the description of the microscopic molecular dynamics of the chondroitin sulfate in natural and artificial cartilage required the identical broad distribution functions for the correlation times of motion, significant differences in the correlation times of motion that are extracted from the model indicate that the artificial tissue does not fully meet the standards of the natural ideal. This could also be confirmed by macroscopic biomechanical elasticity measurements. Nevertheless, these results suggest that NMR is a useful tool for the investigation of the quality of artificially engineered tissue. © 2010 Wiley Periodicals, Inc. Biopolymers 93:520–532, 2010.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com