Matrix metalloproteinase-9 is elevated in serum of patients with amyotrophic lateral sclerosis
Matrix metalloproteinase-9 is elevated in serum of patients with amyotrophic lateral sclerosis
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DOI：
10.1097/00001756-200011090-00003
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发表时间：
2000-11-09
期刊：
影响因子：
1.7
通讯作者：
Weber, F
中科院分区：
文献类型：
--
作者：
Beuche, W;Yushchenko, M;Weber, F
通过酶联免疫吸附测定 (ELISA) 和酶谱法对肌萎缩侧索硬化症 (ALS) 患者的血清和脑脊液 (CSF) 中的基质金属蛋白酶-9 (MMP-9) 及其特异性抑制剂、基质金属蛋白酶-1 (TIMP-1) 组织抑制剂进行分析。与炎症性疾病患者相比，ALS 患者脑脊液中 MMP-9 水平没有升高。然而，与健康供体相比，血清中的 MMP-9 显着升高 (p=0.0003)，达到与病毒性脑膜脑炎 (VM) 或细菌性脑膜炎一样高的水平。 （BM）患者。在对 ALS 患者进行长期观察期间，MMP-9 水平持续升高。在没有炎症反应的情况下，结果表明，ALS患者血清中MMP-9的增加可能是由于失神经肌肉或运动神经元丧失后退化的周围神经中MMP-9的上调所致。 NeuroReport 11:3419-3422 (C) 2000 Lippincott Williams & Wilkins。
Matrix metalloproteinase-9 (MMP-9) and its specific inhibitor, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), were analysed by enzyme-linked immunosorbent assay (ELISA) and by zymography in serum and cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS). In contrast to patients with inflammatory diseases, MMP-9 levels were not elevated in CSF of ALS patients. In serum, however, compared to healthy donors, MMP-9 was significantly (p=0.0003) increased up to levels as high as those of viral meningoencephalitis (VM) or bacterial meningitis. (BM) patients. MMP-9 levels remained elevated during long-term observation of ALS patients. In the absence of an inflammatory response, the results indicate that the increase of MMP-9 in serum of ALS patients might be caused by upregulation of MMP-9 in denervated muscles or in degenerating peripheral nerves following motor neurone loss. NeuroReport 11:3419-3422 (C) 2000 Lippincott Williams & Wilkins.