Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages.
Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages.
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重组人造血生长因子靶标的无血清培养分析：白细胞介素 3 和粒细胞/巨噬细胞集落刺激因子对早期发育阶段具有特异性。
DOI：
10.1073/pnas.85.12.4360
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发表时间：
1988
影响因子：
11.1
通讯作者：
Ogawa,M
中科院分区：
文献类型：
--
作者：
Sonoda,Y;Yang,YC;Wong,GG;Clark,SC;Ogawa,M
我们利用无血清培养系统富集人造血祖细胞，分析了人造血集落刺激因子的发育阶段和谱系特异性。没有一个单独的因素能有效地支持造血集落的形成。白细胞介素3和粒细胞/巨噬细胞集落刺激因子单独或联合使用均不能有效地支持祖细胞的增殖。然而，当与粒细胞集落刺激因子或促红细胞生成素联合使用时，这些因子分别产生中性粒细胞集落或红细胞爆发。白细胞介素3支持培养的连续观察显示，细胞簇的顺序出现和随后的退化。这些观察结果表明，白细胞介素3和粒细胞/巨噬细胞集落刺激因子的主要靶点是发育早期的多能祖细胞，而不是成熟末期的细胞。
We have used a serum-free culture system for enriched human hemopoietic progenitors to analyze the developmental stages and lineage specificities of the human hemopoietic colony-stimulating factors. None of the individual factors alone efficiently supported hemopoietic colony formation. Neither interleukin 3 nor granulocyte/macrophage-colony-stimulating factor alone or in combination effectively supported proliferation of progenitor cells. However, when combined with granulocyte-colony-stimulating factor or erythropoietin, these factors yielded neutrophil colonies or erythroid bursts, respectively. Serial observations of interleukin 3-supported cultures revealed sequential emergence and subsequent degeneration of clusters of cells. These observations suggest that the primary targets of interleukin 3 and granulocyte/macrophage-colony-stimulating factor are multipotent progenitors at the early stages of development rather than cells in the terminal process of maturation.