Global Transcriptional, Physiological, and Metabolite Analyses of the Responses of Desulfovibrio vulgaris Hildenborough to Salt Adaptation
Global Transcriptional, Physiological, and Metabolite Analyses of the Responses of Desulfovibrio vulgaris Hildenborough to Salt Adaptation
复制标题
DOI：
10.1128/aem.02141-09
复制
发表时间：
2010-03-01
影响因子：
4.4
通讯作者：
Zhou, Jizhong
中科院分区：
文献类型：
--
作者：
He, Zhili;Zhou, Aifen;Zhou, Jizhong
通过生理、整体转录和代谢物分析，研究了普通硫弧菌Hildenborough对盐适应(长期的氯化钠暴露)的响应。参与氨基酸生物合成和运输、电子传递、氢氧化和一般胁迫反应的基因(如热休克蛋白、噬菌体休克蛋白和氧化应激反应蛋白)的表达增加，反映了盐的适应。与碳代谢、细胞生长和噬菌体结构相关的基因表达减少。比较了普通杜鹃对盐适应的转录组图谱和普通杜鹃对盐激(短期盐胁迫)的转录组图谱。代谢物分析表明，在盐适应条件下，谷氨酸和丙氨酸积累，提示这两种氨基酸可能作为渗透保护剂用于小球藻。在生长介质中添加氨基酸(谷氨酸、丙氨酸和色氨酸)或酵母膏可以缓解盐相关的生长抑制。提出了一个概念模型，将观察结果与当前可用的知识联系起来，以增加我们对普通杜鹃适应高盐水平的机制的理解。
The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by performing physiological, global transcriptional, and metabolite analyses. Salt adaptation was reflected by increased expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). The expression of genes involved in carbon metabolism, cell growth, and phage structures was decreased. Transcriptome profiles of D. vulgaris responses to salt adaptation were compared with transcriptome profiles of D. vulgaris responses to salt shock (short-term NaCl exposure). Metabolite assays showed that glutamate and alanine accumulated under salt adaptation conditions, suggesting that these amino acids may be used as osmoprotectants in D. vulgaris. Addition of amino acids (glutamate, alanine, and tryptophan) or yeast extract to the growth medium relieved salt-related growth inhibition. A conceptual model that links the observed results to currently available knowledge is proposed to increase our understanding of the mechanisms of D. vulgaris adaptation to elevated NaCl levels.