Mice with only rat mtDNA are required as models of mitochondrial diseases
Mice with only rat mtDNA are required as models of mitochondrial diseases
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DOI：
10.1006/bbrc.2001.4646
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发表时间：
2001-04-06
影响因子：
3.1
通讯作者：
Hayashi, JI
中科院分区：
文献类型：
--
作者：
Yamaoka, M;Mikami, T;Hayashi, JI
我们使用小鼠mtDNA缺失（rho(0)）细胞作为mtDNA受体，研究了通过引入来自不同物种的外源mtDNA来产生表达线粒体功能障碍的小鼠的可能性。为了确定遗传上遥远的mtDNA物种如何在仅具有小鼠核基因组的细胞中复制，我们将叙利亚仓鼠（Mesocricetus auratus）的mtDNA引入小鼠rho(0)细胞，发现其复制不足以繁殖后代，可能是由于小鼠核基因组和叙利亚仓鼠线粒体基因组之间存在显着的不相容性。在小鼠细胞中稳定繁殖并表达线粒体功能障碍的mtDNA，在外源引入小鼠mtDNA后也迅速消失，提示在引入大鼠mtDNA之前，必须完全排除小鼠细胞中的小鼠mtDNA，才能产生以大鼠mtDNA作为线粒体疾病模型的小鼠。(C) 2001学术出版社。
We examined the possibility of generation of mice expressing mitochondrial dysfunction by introduction of exogenous mtDNA from different species using mouse mtDNA-less (rho (0)) cells as mtDNA recipients. For determination of how genetically distant species of mtDNA could replicate in cells with only the mouse nuclear genome, we introduced mtDNA of the Syrian hamster (Mesocricetus auratus) into mouse rho (0) cells, and found that its replication was not sufficient to propagate to following generations, probably due to significant incompatibility between mouse-nuclear and Syrian hamster-mitochondrial genomes, On the other hand, rat mtDNA, which propagated stably and expressed mitochondrial dysfunction in mouse cells, also disappeared rapidly by exogenous introduction of mouse mtDNA, suggesting that mouse mtDNA in mouse cells must be excluded completely before introduction of rat mtDNA for generation of mice with rat mtDNA as mitochondrial disease models. (C) 2001 Academic Press.