Expression of endocrine genes in zebrafish larvae in response to environmental salinity
Expression of endocrine genes in zebrafish larvae in response to environmental salinity
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DOI：
10.1677/joe-07-0003
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发表时间：
2007-06-01
影响因子：
4
通讯作者：
Hirose, Shigehisa
中科院分区：
文献类型：
--
作者：
Hoshijima, Kazuyuki;Hirose, Shigehisa
我们测试了斑马鱼内分泌系统在幼虫阶段应对环境盐分挑战的能力。我们发现，斑马鱼幼虫有一个系统，其中多个内分泌基因，包括心房钠尿肽（anp）、肾素、催乳素、生长激素（gh）和甲状旁腺激素1（pth 1），在转录水平上对环境盐度的变化做出反应，并且这些反应是基因特异性的。在稀淡水培养基中饲养的幼虫中，anp 和肾素均上调，但在浓缩培养基中则下调。另一方面，催乳素和 gh 的表达在稀培养基中强烈增强，但在较高盐度条件下几乎没有变化。有趣的是，正如在哺乳动物中观察到的那样，PTH1 表达取决于 Ca2+ 浓度。因此，结合模型生物的优势，包括遗传方法的可及性，我们认为斑马鱼幼虫可用于全面研究内分泌系统在离子和渗透稳态的调节机制。
We tested the capability of the endocrine system in zebrafish to respond to environmental salinity challenges during larval stages. We reveal that the zebrafish larvae have a system in which several endocrine genes, including atrial natriuretic peptide (anp), renin, prolactin,growth hormone (gh) and parathyroid hormone 1 (pth 1), respond at the transcription level to changes in environmental salinity and that the responses are gene specific. Both anp and renin are upregulated in larvae raised in dilute freshwater medium but are downregulated in concentrated medium. On the other hand, expression of prolactin and gh is strongly enhanced in the dilute medium, but shows little or no change under higher salinity conditions. Interestingly, PTH1 expression depends on Ca2+ concentration, as observed in mammals. Thus, taken together with the advantages of a model organism, including accessibility to genetic approaches, we propose that zebrafish larvae are useful for a comprehensive study of the regulatory mechanisms of the endocrine system in ionic and osmotic homeostasis.