BLOOD-ALCOHOL CONCENTRATION AND SEVERITY OF MICROENCEPHALY IN NEONATAL RATS DEPEND ON THE PATTERN OF ALCOHOL ADMINISTRATION
BLOOD-ALCOHOL CONCENTRATION AND SEVERITY OF MICROENCEPHALY IN NEONATAL RATS DEPEND ON THE PATTERN OF ALCOHOL ADMINISTRATION
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DOI：
10.1016/0741-8329(88)90054-7
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发表时间：
1988-05-01
期刊：
影响因子：
2.3
通讯作者：
WEST, JR
中科院分区：
文献类型：
--
作者：
BONTHIUS, DJ;GOODLETT, CR;WEST, JR
孕晚期胎儿酒精暴露的大鼠模型被用来研究如何管理模式的每日酒精剂量的影响模式的血液酒精浓度（BAC）和大脑生长受限的严重程度。四组大鼠从出生后第4天到第10天人工饲养。其中三组接受相同的每日剂量的酒精（6.6 g/kg/天），但每日模式不同。在一组中，剂量以2.5%（v/v）溶液连续给药;在另外两组中，剂量浓缩为7.5%或15.0%（v/v）溶液。第四组（胃痛对照组）接受含有麦芽糖糊精的配方，其与2.5%酒精溶液等热量。每日两次测定BAC，时间设计为估计每日峰值和最小BAC。出生后第10天处死大鼠，最大BAC出现在出生后第6天下午。此后，每日峰值BAC逐渐下降。酒精给药的模式越集中，达到的最大BAC就越高，对大脑生长的干扰就越严重。虽然相对于胃造口术对照组，以小的连续部分（2.5%）接受酒精剂量的组未表现出任何显著的微脑畸形，但以更浓缩形式（7.5%和15.0%）接受剂量的组表现出显著的脑生长受限（相对于对照组，分别减少10%和31%）。 因此，对于给定的每日剂量的酒精，峰值BAC和酒精诱导的脑生长限制的严重程度取决于酒精给药的模式。
A rat model of third trimester fetal alcohol exposure was used to examine how the pattern of administration of a daily alcohol dose influences the pattern of blood alcohol concentrations (BACs) and the severity of brain growth restriction. Four groups of rats were artifically reared from postnatal days 4 to 10. Three of the groups received an equivalent daily dose of alcohol (6.6 g/kg/day) but in different daily patterns. To one group, the dose was administered continously in a 2.5% (v/v) solution; in two other groups the dose was condensed into either 7.5% or 15.0% (v/v) solutions. A fourth group (gastrosmy controls) received a formula containing maltose-dextrin, which was isocaloric to the 2.5% alcohol solution. BACs were determined twice daily at times designed to estimate the daily peak and minimum BACs. The rats were killed on postnatal day 10 and maximum BAC occurred on the afternoon of postnatal day 6. Thereafter, daily peak BAC declined progressively. The more concentrated the pattern of alcohol administration, the higher was the maximum BAC achieved and the more severe was the interference with brain growth. While the group receiving the alcohol dose in small continuous fractions (2.5%) did not exhibit any significant microencephaly, relative to gastrostomy controls, the groups receiving the dose in more concentrated forms (7.5% and 15.0%)exhibited significant brain growth restriction (reduced 10% and 31% respectively, relative to controls. Therefore, the peak BAC and the severity of the alcohol-induced brain growth restriction for a given daily dose of alcohol depend on the pattern of alcohol adminstration.