THE PURIFICATION OF MEGAPOIETIN - A PHYSIOLOGICAL REGULATOR OF MEGAKARYOCYTE GROWTH AND PLATELET PRODUCTION
THE PURIFICATION OF MEGAPOIETIN - A PHYSIOLOGICAL REGULATOR OF MEGAKARYOCYTE GROWTH AND PLATELET PRODUCTION
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DOI：
10.1073/pnas.91.23.11104
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发表时间：
1994-11-08
影响因子：
11.1
通讯作者：
ROSENBERG, RD
中科院分区：
文献类型：
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作者：
KUTER, DJ;BEELER, DL;ROSENBERG, RD
循环血液中的血小板是由骨髓巨核细胞产生的。随着血小板计数的减少，巨核细胞的数量和倍性增加。虽然这种反馈回路一直被认为是由循环造血因子介导的，但没有这种因子被纯化。利用绵羊血小板减少症模型，我们发现了一种被称为巨生成素的活性物质，它刺激骨髓培养中巨核细胞数量和倍性的增加。这种因子的循环水平可以用这种方法量化，并且发现它与绵羊的血小板计数成反比。水平从
The circulating blood platelet is produced by the bone marrow megakaryocyte. In response to a decrease in the platelet count, megakaryocytes increase in number and ploidy. Although this feedback loop has long been thought to be mediated by a circulating hematopoietic Factor, no such factor has been purified. Using a model of thrombocytopenia in sheep, we have identified an active substance called megapoietin, which stimulated an increase in the number and ploidy of megakaryocytes in bone marrow culture. Circulating levels of this factor could be quantified with this assay and were found to be inversely proportional to the platelet count of the sheep. Levels increased from