Development of mediator-type biosensor to wirelessly monitor whole cholesterol concentration in fish
Development of mediator-type biosensor to wirelessly monitor whole cholesterol concentration in fish
复制标题
开发介质型生物传感器来无线监测鱼中的总胆固醇浓度
DOI：
10.1007/s10695-013-9851-1
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
H. Endo
中科院分区：
文献类型：
--
作者：
M. Takase;M. Murata;K. Hibi;R. Huifeng;H. Endo
我们开发了一种无线监测系统，通过跟踪总胆固醇浓度的变化来监测鱼的状况。鱼类的总胆固醇浓度是类固醇激素的来源或免疫水平的指标，这使得其检测对于跟踪鱼类的生理状况具有重要意义。无线监测系统由介体型生物传感器和无线传输装置组成。生物传感器植入鱼体，传输装置轻便，监测时鱼可以自由游动。将胆固醇酯酶和氧化酶固定在生物传感器的检测位点上，用于检测总胆固醇浓度。然而，胆固醇氧化酶利用氧的氧化还原反应进行检测，其浓度容易因环境条件的变化而波动。同时，介体型生物传感器可以利用介体代替氧的氧化还原反应来监测总胆固醇浓度。测试了制作的介体型生物传感器的特性。在0-8 ppm的氧气浓度波动下，介体型生物传感器的传感器输出电流与非介体型生物传感器的输出电流相比保持稳定，这意味着该传感器受溶解氧浓度变化的影响较小。然后将该生物传感器植入鱼体内进行无线监测。结果，大约 48 小时的实时监测取得了成功。
We developed a wireless monitoring system to monitor fish condition by tracking the change in whole cholesterol concentration. The whole cholesterol concentration of fish is a source of steroid hormones or indicator of immunity level, which makes its detection important for tracking physiological condition of fish. Wireless monitoring system comprises of mediator-type biosensor and wireless transmission device. Biosensor is implantable to fish body, and transmission device is so light, in that fish is allowed to swim freely during monitoring. Cholesterol esterase and oxidase were fixated on to the detection site of biosensor and used to detect the whole cholesterol concentration. However, cholesterol oxidase incorporates oxidation–reduction reaction of oxygen for detection, which concentration fluctuates easily due to change in environmental condition. Meanwhile, mediator-type biosensor enables monitoring of whole cholesterol concentration by using mediator to substitute that oxidation–reduction reaction of oxygen. Characteristic of fabricated mediator-type biosensor was tested. The sensor output current of mediator-type biosensor remained stable compared to output current of non-mediator-type biosensor under fluctuating oxygen concentration of 0–8 ppm, which implied that this sensor is less affected by change in dissolved oxygen concentration. That biosensor was then implanted into fish for wireless monitoring. As a result, approximately 48 h of real-time monitoring was successful.