Non-invasive imaging of ion concentration distribution around cell spheroids by electrical impedance tomographic sensor printed on circuit board under temporal compensation by ion transport impedance model
Non-invasive imaging of ion concentration distribution around cell spheroids by electrical impedance tomographic sensor printed on circuit board under temporal compensation by ion transport impedance model
复制标题
在离子传输阻抗模型的时间补偿下，通过印刷在电路板上的电阻抗断层扫描传感器对细胞球体周围的离子浓度分布进行非侵入性成像
DOI：
10.1016/j.bios.2022.114432
复制
发表时间：
2022
影响因子：
12.6
通讯作者：
Takei Masahiro
中科院分区：
文献类型：
--
作者：
Kawashima Daisuke;Yuki Tsubasa;Li Songshi;Takei Masahiro
提出了一种称为PCB-EIT成像的非侵入性成像，通过印刷在电路板上的新开发的电阻抗断层扫描传感器（PCB-EIT传感器）以0.1秒的时间分辨率对细胞球体周围的时空离子浓度分布进行成像。为了实现PCB-EIT成像的高时间分辨率，采用了基于Cole-Cole方程的细胞外溶液电阻Rex的离子输运阻抗模型进行时间补偿。为了确认PCB-EIT成像的性能，在MRC-5的细胞质和细胞核中表达的绿色荧光蛋白导致电性不同的三种细胞球状体类型（GFP型（GFPT）、组蛋白型（HT）和野生型（WT））周围的离子浓度分布。结果表明，PCB-EIT成像成功地重建了细胞球体离子输运引起的离子浓度时空分布。在分布误差εdisof 0.046 ± 0.0038的范围内，PCB-EIT成像图像与荧光比值成像图像的对比结果得到了验证。对于通过基于Fick定律的传质模拟来评估每种细胞球体类型的离子扩散率Dm，GFPT的Dm在4 s的较早时间范围内显示出三种细胞类型中的最高值，而HT的Dm在15 s的时间范围内显示出最高值，这表明PCB-EIT成像能够评估每种细胞类型的离子传输特性。
A non-invasive imaging called as PCB-EIT imaging has been proposed in order to image spatio-temporal ion concentration distribution around cell spheroids in 0.1 s of temporal resolution by a newly developed electrical impedance tomographic sensor printed on circuit board (PCB-EIT sensor). To realize the high temporal resolution in PCB-EIT imaging, the temporal compensation by the ion transport impedance model interpolating the extracted resistance of extracellular solutionRexobtained from the Cole-Cole equation is employed. To confirm the performance of PCB-EIT imaging, the ion concentration distribution around three cell spheroid types (GFP type (GFPT), Histone type (HT) and wild type (WT)) which are electrically different due to green fluorescent protein expressed in cytoplasm and nucleus of MRC-5. As a result, spatio-temporal ion concentration distributions due to ion transport from cell spheroid are successfully reconstructed by PCB-EIT imaging. The images by PCB-EIT imaging are validated with those by fluorescence ratio imaging within the distribution errorεdisof 0.046 ± 0.0038 in maximum. For an evaluation of the ion diffusivityDmof each cell spheroid type by the mass transfer simulation based on Fick’s law,Dmof GFPT shows the highest value among the three cell types in the earlier time range from 4 s, whileDmof HT shows the highest one in the time range from 15 s, which indicates that PCB-EIT imaging is able to evaluate the ion transport characteristics of each cell type.