DNA-BINDING SITE OF THE GROWTH FACTOR-INDUCIBLE PROTEIN ZIF268
DNA-BINDING SITE OF THE GROWTH FACTOR-INDUCIBLE PROTEIN ZIF268
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DOI：
10.1073/pnas.86.22.8737
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
NATHANS, D
中科院分区：
文献类型：
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作者：
CHRISTY, B;NATHANS, D
Zif268 是一种锌指蛋白，其 mRNA 在暴露于生长因子或其他信号剂的细胞中迅速激活，被认为在调节细胞外配体诱导的遗传程序中发挥作用。我们报道Zif268具有转录调节因子的特征之一，即与DNA的序列特异性结合。在大肠杆菌中合成的 Zif268 与 zip268 基因上游的两个位点以及其他基因启动子区域的位点结合。通过 DNase I 足迹、甲基化干扰实验以及合成寡核苷酸的使用来定义负责结合的核苷酸序列。从这些结果中，我们得出了 Zif268 高亲和力结合位点的以下共有序列：GCGTGGGGCG。
Zif268, a zinc finger protein whose mRNA is rapidly activated in cells exposed to growth factor or other signaling agents, is thought to play a role in regulating the genetic program induced by extracellular ligands. We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence-specific binding to DNA. Zif268 synthesized in Escherichia coli bound to two sites upstream of the zip268 gene and to sites in the promoter regions of other genes. The nucleotide sequences responsible for binding were defined by DNase I footprinting, by methylation interference experiments, and by use of synthetic oligonucleotides. From these results we derived the following consensus sequence for a Zif268 high-affinity binding site: GCGTGGGGCG.