ACTIVATION OF INFECTIOUS VIRUS FROM LATENT HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION OF MONOCYTES INVIVO
ACTIVATION OF INFECTIOUS VIRUS FROM LATENT HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION OF MONOCYTES INVIVO
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DOI：
10.1172/jci116016
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发表时间：
1992-10-01
影响因子：
15.9
通讯作者：
RUSCETTI, FW
中科院分区：
文献类型：
--
作者：
MIKOVITS, JA;LOHREY, NC;RUSCETTI, FW
感染艾滋病毒的人在发展为明显的艾滋病之前可能会多年没有症状。由于HIV可以潜伏感染单核细胞系，我们检查了体内单核细胞是否存在HIV潜伏感染。从培养前后检查的无症状血清阳性个体新鲜分离的单核细胞中，HIV DNA呈阳性，但聚合酶链式反应检测不到RNA，表明HIV潜伏在体内的单核细胞中。这些潜伏感染的单核细胞与来自HIV阴性的正常捐赠者的ConA激活的T细胞共培养，刺激了90%的患者样本，并潜伏感染THP-1以产生传染性病毒。Con A、静息T细胞和T细胞培养上清液均不能诱导病毒。激活的T细胞的质膜刺激艾滋病毒的产生，这表明细胞接触诱导单核细胞中的因子(S)可以克服潜伏期。因此，艾滋病患者的单核细胞在免疫反应中含有潜在的可诱导的HIV，导致T细胞感染和病毒诱导的病理。
Individuals infected with HIV may be asymptomatic for years before progressing to overt AIDS. Since HIV can latently infect monocytoid cell lines, we examined whether HIV latency occurs in monocytes in vivo. Freshly isolated monocytes from asymptomatic seropositive individuals examined before and after culture were positive for HIV DNA, but not RNA, as measured by polymerase chain reaction, showing that HIV latency occurs in monocytes in vivo. Coculture of these latently infected monocytes with Con A-activated T cells from HIV-negative normal donors stimulated 90% of the patients' samples and latently infected THP-1 to produce infectious virus. Neither Con A, resting T cells, nor T cell supernatants induced virus. Plasma membranes from activated T cells stimulated HIV production, suggesting cell contact induces factor(s) in monocytes to overcome latency. Thus, monocytes in AIDS patients harbor latent HIV inducible during an immune response, leading to T cell infection and viral-induced pathology.