Comparative Analysis of a Series of pH-Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification
Comparative Analysis of a Series of pH-Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification
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一系列 pH 响应型磺化铕配合物用于生物测定监测酸化的比较分析
DOI：
10.1002/ejic.202200426
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发表时间：
2022
影响因子：
2.3
通讯作者：
Fradgley J
中科院分区：
文献类型：
--
作者：
Fradgley J
报道了一系列亲水性磺化和 pH 响应发光铕 (III) 探针的合成和光物理评价。当 pH 值降低时，Eu 的发射强度和寿命显着增加，导致 Eu 发光在酸性介质中“开启”。通过改变位于敏化发色团中的完整苯胺氮原子上的取代基的性质，对于一组五个Eu(III)络合物，络合物的pKa值可以调整到4.3和6.3之间。这些系统被设计为允许与靶向载体缀合，从而允许例如监测细胞中的受体内化。这种方法可用于选择性地标记蛋白质，并允许监测酸性细胞器的摄取，从而能够创建时间分辨内化测定，以实时跟踪体外和细胞内的 pH 变化。
The synthesis and photophysical evaluation of a series of hydrophilic sulphonated and pH responsive luminescent europium(III) probes are reported. The Eu emission intensities and lifetimes increase very significantly on lowering pH, leading to a ‘switching on’ of Eu luminescence in more acidic media. By varying the nature of the substituents at an integral aniline nitrogen atom located in the sensitising chromophore, the pKavalue of the complex can be tuned to between 4.3 and 6.3 for a set of five Eu(III) complexes. These systems are designed to allow conjugation to targeting vectors permitting, for example, the monitoring of receptor internalisation in cells. This approach can be used to tag proteins selectively and allows the monitoring of uptake into acidic organelles, enabling the creation of time‐resolved internalisation assays to follow pH change in real time, both in vitro and in cellulo.