Effects of dihydrotestosterone administration on the expression of reproductive and body weight regulatory factors in ovariectomized and estradiol-treated female rats
Effects of dihydrotestosterone administration on the expression of reproductive and body weight regulatory factors in ovariectomized and estradiol-treated female rats
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DOI：
10.1080/09513590.2017.1337096
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Irahara, Minoru
中科院分区：
文献类型：
--
作者：
Iwasa, Takeshi;Matsuzaki, Toshiya;Irahara, Minoru
为了阐明雄激素的直接影响，我们在雌性大鼠中评估了长期使用二氢睾酮（DHT）引起的下丘脑生殖和食欲调节因子水平的变化。 DHT 治疗增加了卵巢切除大鼠的体重和食物摄入量，但雌二醇 (E2) 治疗的大鼠没有增加。 DHT 给药抑制了下丘脑厌食因子的表达。尽管 E2 治疗的大鼠下丘脑前部阻滞（前腹侧室周核，AVPV）的 Kisspeptin (Kiss1) mRNA 水平增加，但 DHT 给药并不影响卵巢切除或 E2 治疗的大鼠 AVPV 的 Kiss1 mRNA 水平。相反，DHT 给药降低了卵巢切除大鼠下丘脑后区（弓状核，ARC）的 Kiss1 mRNA 水平。尽管 E2 治疗的大鼠下丘脑后阻滞 (ARC) 的 Kiss1 mRNA 水平降低，但 DHT 给药并不影响这些大鼠中 ARC 的 Kiss1 mRNA 水平。这些组的血清黄体生成激素水平表现出与 ARC 的 Kiss1 mRNA 水平相似的模式。这些结果表明，DHT 影响下丘脑生殖和食欲调节因子的产生，并且 DHT 的这些影响根据雌激素环境的不同而不同。
To clarify the direct effects of androgens, the changes in the hypothalamic levels of reproductive and appetite regulatory factors induced by chronic dihydrotestosterone (DHT) administration were evaluated in female rats. DHT treatment increased the BW and food intake of the ovariectomized rats, but not the estradiol (E2)-treated rats. DHT administration suppressed the expression of a hypothalamic anorexigenic factor. Although the kisspeptin (Kiss1) mRNA levels of the anterior hypothalamic block (the anteroventral periventricular nucleus, AVPV) were increased in the E2-treated rats, DHT administration did not affect the Kiss1 mRNA levels of the AVPV in the ovariectomized or E2-treated rats. Conversely, DHT administration reduced the Kiss1 mRNA levels of the posterior hypothalamic block (the arcuate nucleus, ARC) in the ovariectomized rats. Although the Kiss1 mRNA levels of the posterior hypothalamic block (ARC) were decreased in the E2-treated rats, DHT administration did not affect the Kiss1 mRNA levels of the ARC in these rats. Serum luteinizing hormone levels of these groups exhibited similar patterns to the Kiss1 mRNA levels of the ARC. These results showed that DHT affects the production of hypothalamic reproductive and appetite regulatory factors, and that these effects of DHT differ according to the estrogen milieu.