Rhizorhabdus dicambivorans sp. nov., a dicamba-degrading bacterium isolated from compost.
Rhizorhabdus dicambivorans sp. nov., a dicamba-degrading bacterium isolated from compost.
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DOI：
10.1099/ijsem.0.001194
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发表时间：
2016-09
影响因子：
2.8
通讯作者：
Li Yao;Jun‐Jie Zhang;Linrui Yu;Qin Chen-;Jian-chun Zhu;Jian He;Derong Ding
中科院分区：
文献类型：
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作者：
Li Yao;Jun‐Jie Zhang;Linrui Yu;Qin Chen-;Jian-chun Zhu;Jian He;Derong Ding
从植物凋落物堆肥中分离到一株革兰氏染色阴性、无芽胞的细菌NDBN-20T。该菌株能够降解麦草畏。基于16S rRNA基因序列的系统发育分析表明，NDBN-20T属于嗜热菌属鞘氨醇单胞菌科成员，与丝氨醇单胞菌SP1T(98.8%)、鞘氨醇单胞菌RW1T(97.9%)、鞘氨醇单胞菌382T(97.7%)和鞘氨醇单胞菌UM2T(97.7%)有较高的同源性。但该菌株的DNA序列与银斑链霉菌SP1T(45.6±1.9%)、维氏链球菌RW1T(33.5±2.3%)、组氨酸链霉菌UM2T(39.4±3.6%)和星伯氏链霉菌382T(42.1±4.1%)的同源性较低。NDBN-20T以Q-10为主，亚精胺为主要多胺，总和特征8(包括C18：1ω7c/C18：1ω6c)，总和特征3(包括C16：1ω7c/C16：1ω6c)，C17：1ω6c，C16：0和C14：02-OH为主要脂肪酸(>；占总数的5%)。极性脂的组成包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、糖脂、鞘糖脂、磷脂酰二甲基乙醇胺和磷脂酰甘油。DNA G+C含量为65.4mol%。在多相分类的基础上，NDBN-20T菌株被认为是根状杆菌属的一个新种，建议命名为双目根状杆菌。11月模式菌株为NDBN-20T(=CCTCC AB 2016143=KACC 18661)。
Strain Ndbn-20T, a Gram-staining-negative, non-spore-forming bacterium, was isolated from compost of plant litter. The strain was able to degrade dicamba. Phylogenetic analysis based on 16S rRNA gene sequences indicated that Ndbn-20Trepresented a member of the family Sphingomonadaceae of the Alphaproteobacteria and showed high sequence similarities to Rhizorhabdusargentea SP1T (98.8 %), Sphingomonaswittichii RW1T (97.9 %), Sphingomonasstarnbergensis 382T (97.7 %) and Sphingomonashistidinilytica UM2T (97.7 %). However, the strain showed low DNA sequence relatedness with R. argentea SP1T (45.6±1.9 %), S. wittichii RW1T (33.5±2.3 %), S.histidinilytica UM2T (39.4±3.6 %) and S. starnbergensis382T (42.1±4.1 %). Ndbn-20T possessed Q-10 as the predominant ubiquinone, spermidine as the major polyamine, and summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), C17 : 1ω6c, C16 : 0 and C14 : 02-OH as the major fatty acids (>5 % of the total). The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, glycolipid, sphingoglycolipid, phosphatidyldimethylethanolamine and phosphatidylglycerol. The DNA G+C content was 65.4 mol%. Based on a polyphasic taxonomic analysis, strain Ndbn-20T is proposed to represent a novel species of the genus Rhizorhabdus, with the proposed name of Rhizorhabdus dicambivorans sp. nov. The type strain is Ndbn-20T (=CCTCC AB 2016143=KACC 18661).