Low-cost photodynamic therapy devices for global health settings: Characterization of battery-powered LED performance and smartphone imaging in 3D tumor models.
Low-cost photodynamic therapy devices for global health settings: Characterization of battery-powered LED performance and smartphone imaging in 3D tumor models.
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用于全球健康环境的低成本光动力疗法：3D肿瘤模型中电池供电的LED性能和智能手机成像的表征。
DOI：
10.1038/srep10093
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发表时间：
2015-05-12
影响因子：
4.6
通讯作者：
Celli JP
中科院分区：
文献类型：
--
作者：
Hempstead J;Jones DP;Ziouche A;Cramer GM;Rizvi I;Arnason S;Hasan T;Celli JP
发达国家和发展中国家之间的全球癌症健康差距涉及在资源有限的环境中无法获得有效的癌症治疗。光动力疗法（PDT）是一种基于光的治疗方式，其在临床中使用由电池供电的发光二极管（LED）可实现的波长和辐照度表现出安全性和有效性。在这里，我们评估了低成本的使能技术，以扩大PDT的临床效益，很少或根本没有获得电力或医疗基础设施的地区。我们证明了一种基于635 nm高输出LED的装置的有效性，该装置由三节AA一次性碱性电池供电，通过给予氨基乙酰丙酸（ALA）诱导原卟啉IX（PpIX）的积累，在光敏化后A431鳞状癌细胞的单层和3D培养物中实现强细胞毒性反应。在这里，我们描述了电池供电设备性能的挑战，包括电池消耗和电压稳定性，特别是在相关的PDT剂量参数。PDT光敏剂作为肿瘤选择性荧光造影剂的成熟能力的进一步激励，我们证明了具有低成本附加组件的消费者智能手机测量浓度依赖性PpIX荧光的能力。这项研究为全球健康应用的图像引导ALA-PDT治疗技术的持续发展奠定了基础。
A lack of access to effective cancer therapeutics in resource-limited settings is implicated in global cancer health disparities between developed and developing countries. Photodynamic therapy (PDT) is a light-based treatment modality that has exhibited safety and efficacy in the clinic using wavelengths and irradiances achievable with light-emitting diodes (LEDs) operated on battery power. Here we assess low-cost enabling technology to extend the clinical benefit of PDT to regions with little or no access to electricity or medical infrastructure. We demonstrate the efficacy of a device based on a 635 nm high-output LED powered by three AA disposable alkaline batteries, to achieve strong cytotoxic response in monolayer and 3D cultures of A431 squamous carcinoma cells following photosensitization by administering aminolevulinic acid (ALA) to induce the accumulation of protoporphyrin IX (PpIX). Here we characterize challenges of battery-operated device performance, including battery drain and voltage stability specifically over relevant PDT dose parameters. Further motivated by the well-established capacity of PDT photosensitizers to serve as tumour-selective fluorescence contrast agents, we demonstrate the capability of a consumer smartphone with low-cost add-ons to measure concentration-dependent PpIX fluorescence. This study lays the groundwork for the on-going development of image-guided ALA-PDT treatment technologies for global health applications.
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影响因子：
12.4
作者：
Glidden MD;Celli JP;Massodi I;Rizvi I;Pogue BW;Hasan T
通讯作者：
Hasan T
影响因子：
3.4
作者：
Pogue, BW;Hasan, T
通讯作者：
Hasan, T
影响因子：
11.2
作者：
Rahmanzadeh, Ramtin;Rai, Prakash;Hasan, Tayyaba
通讯作者：
Hasan, Tayyaba
影响因子：
3.5
作者：
Anbil, Sriram;Rizvi, Imran;Hasan, Tayyaba
通讯作者：
Hasan, Tayyaba
DOI：
10.1016/1011-1344(90)85083-9
发表时间：
1990-06-01
影响因子：
5.4
作者：
KENNEDY, JC;POTTIER, RH;PROSS, DC
通讯作者：
PROSS, DC