Neuronal control of pedal sole cilia in the pond snail Lymnaea stagnalis appressa
Neuronal control of pedal sole cilia in the pond snail Lymnaea stagnalis appressa
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DOI：
10.1007/s00359-012-0770-x
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发表时间：
2013-01-01
影响因子：
2.1
通讯作者：
Peterman, Misa
中科院分区：
文献类型：
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作者：
Longley, Roger D.;Peterman, Misa
5-HT（血清素）是一种普遍存在的神经递质，局部使用时会在腹足动物中产生纤毛跳动，但仅在三对神经元中描述了由腹足动物血清素能神经元引起的纤毛跳动。我们将这些结果扩展到北美 Lymnaea stagnalis appressa，它是与欧洲 Lymnaea stagnalis 不同的物种。我们描述了一对非血清素能神经元 PeV1，它加速足底粘膜纤毛运输；还有一对血清素能神经元 PeD7，它减慢粘液纤毛运输。我们比较和讨论了 L. s. 的发育和识别的神经元。 appressa 和 L. stagnalis 中，它们与 L. s. 有同源物。贴压 PeD7 和 PeV1 神经元。除了 PeD7 和 PeV1 神经元之外，我们还测试了对 Tritonia 踏板肽抗体具有免疫反应的神经元，粘液纤毛转运结果为阴性。在表征 PeD7 和 PeV1 神经元时，我们发现 PeV1 不会激发 PeD7。在半完整制剂中，PeD7 神经元活性在触觉刺激期间发生强烈增加，但 V1 神经元在触觉刺激期间受到抑制。触觉刺激后，PeV1 神经元表现出强烈的活性。这表明两个神经元对的功能存在明显差异，这两个神经元对的轴突都覆盖在足底睫状细胞上。将 5-HT 应用于踏板底会在 1.4-1.9 秒内启动粘膜纤毛运输，其时间过程与刺激 PeV1 神经元时所见的时间过程相似。这一结果似乎是通过踏板底上的 5-HT1A 样受体获得的。我们描述了踏板底上 5-HT 的可能外部来源，来自随粘液释放的 5-HT 免疫反应颗粒。
5-HT (serotonin) is a ubiquitous neurotransmitter that produces ciliary beating in gastropods when applied topically, but ciliary beating caused by gastropod serotonergic neurons has been described in only three neuron pairs. We extend these results to the North American Lymnaea stagnalis appressa, which is a different species from the European Lymnaea stagnalis. We describe a non-serotonergic neuron pair, PeV1, which accelerates pedal sole mucociliary transport and a serotonergic neuron pair, PeD7, which slows mucociliary transport. We compare and discuss development and identified neurons in L. s. appressa and in L. stagnalis, which have homologs to L. s. appressa PeD7 and PeV1 neurons. In addition to PeD7 and PeV1 neurons, we test neurons immunoreactive to Tritonia pedal peptide antibodies with negative results for mucociliary transport. In characterizing PeD7 and PeV1 neurons, we find that PeV1 does not excite PeD7. In semi-intact preparations, a strong increase in PeD7 neuron activity occurs during tactile stimulation, but V1 neurons are inhibited during tactile stimulation. Following tactile stimulation, PeV1 neurons show strong activity. This suggests a distinct difference in function of the two neuron pairs, which both have their axons overlying pedal sole ciliary cells. Application of 5-HT to the pedal sole initiates mucociliary transport in 1.4-1.9 s with a time course similar to that seen when stimulating a PeV1 neuron. This result appears to be through a 5-HT1A-like receptor on the pedal sole. We describe a possible external source of 5-HT on the pedal sole from 5-HT immunoreactive granules that are released with mucus.