Antibacterial effects of levofloxacin, erythromycin, and rifampin in a human monocyte system against Legionella pneumophila
Antibacterial effects of levofloxacin, erythromycin, and rifampin in a human monocyte system against Legionella pneumophila
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DOI：
10.1128/aac.42.12.3153
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发表时间：
1998-12-01
影响因子：
4.9
通讯作者：
Michelsen, PB
中科院分区：
文献类型：
--
作者：
Baltch, AL;Smith, RP;Michelsen, PB
研究了左氧氟沙星、红霉素和利福平对细胞内嗜肺军团菌 L-1033（血清型 1）的抗菌活性。在使用贴壁人单核细胞、嗜肺军团菌 L-1033、吞噬时间为 1 小时、抗生素（左氧氟沙星、红霉素和/或利福平）为 MIG 的 1 至 10 倍的体外系统中，在 0 至 4 天的时间段内测定单核细胞裂解物的 CFU/ml 值。 pH 7.4 时，左氧氟沙星的 CFU/ml 下降迅速，发生在 24 小时内，并且与药物浓度相关（P < 0.01）。利福平的 CFU 下降在 48 小时首次观察到（P < 0.01），而红霉素仅观察到 CFU/ml 的最小下降。红霉素在其 MIC 十倍的情况下显着降低了 CFU/ml 值（P < 0.01），达到单独使用左氧氟沙星所达到的值，而利福平和红霉素的组合则没有。孵育 24 小时后去除左氧氟沙星导致嗜肺军团菌 L-1033 重新生长，而去除利福平后观察到 CFU/ml 持续缓慢下降； CFU/ml 值不受红霉素去除的影响。4 天时，甚至在去除抗生素后进行的测定中，左氧氟沙星和利福平测定中的 CFU/ml 值仍然低于红霉素测定中的 CFU/ml 值。左氧氟沙星对嗜肺军团菌 L-1033 的杀菌活性明显高于红霉素或利福平。在这些测定中，添加红霉素或利福平会死！不影响左氧氟沙星的抗菌活性。
The antibacterial activities of levofloxacin, erythromycin, and rifampin against intracellular Legionella pneumophila L-1033, serogroup 1, were studied. In an in vitro system utilizing adherent human monocytes, L. pneumophila L-1033, a phagocytosis time period of 1 h, and antibiotic (levofloxacin, erythromycin, and/or rifampin) at 1 to 10 times the MIG, the CFU/ml values for the monocyte lysate were determined during 0- to 4-day time periods. The decrease in CFU/ml with levofloxacin at pH 7.4 was rapid, occurring within 24 h, and was drug concentration dependent (P < 0.01), The decrease in CFU with rifampin was first observed at 48 h (P < 0.01), while only a minimal decrease in CFU/ml was observed with erythromycin, Combination of levofloxacin and rifampin and of levofloxacin and erythromycin at ten times their MICs significantly decreased the CFU/ml value (P < 0.01), to the value attained by levofloxacin alone, while combination of rifampin and erythromycin did not. Removal of levofloxacin after 24 h of incubation resulted in regrowth of L. pneumophila L-1033, while a continued slow decrease in CFU/ml was seen following rifampin removal; CFU/ml values were unaffected by the removal of erythromycin, At 4 days, and even in assays performed following antibiotic removal, the CFU/ml value continued to be lower in the levofloxacin and rifampin assays than in the assays with erythromycin. Levofloxacin had a significantly higher bactericidal activity against L. pneumophila L-1033 than erythromycin or rifampin, In these assays, the addition of erythromycin or rifampin die! not affect the antibacterial activity of levofloxacin.