GENOTOXICITY ASSAY OF 5 PESTICIDES AND THEIR MIXTURES IN SACCHAROMYCES-CEREVISIAE D7
GENOTOXICITY ASSAY OF 5 PESTICIDES AND THEIR MIXTURES IN SACCHAROMYCES-CEREVISIAE D7
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DOI：
10.1016/0165-1218(94)90071-x
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发表时间：
1994-06-01
期刊：
影响因子：
--
通讯作者：
CHIESARA, E
中科院分区：
文献类型：
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作者：
BIANCHI, L;ZANNOLI, A;CHIESARA, E
在有无S9代谢系统的情况下，对4种有机磷农药（甲基氮磷、二嗪酮、乐果酯和甲基吡啶磷）和1种氨基甲酸酯（苯甲酰）在酿酒酵母菌D7中的细胞毒性、反向突变和基因转化进行了测试。此外，在同一实验模型中，对上述化合物的两种混合物，即苯甲酰+吡嗪磷-甲基（6/1）和乐果+重氮酮+吡嗪磷-甲基（10/4/6）进行了测试。单Azinphos-methyl、苯甲酰和pirimiphos-methyl均无基因毒性作用，而Azinphos-methyl和diazinone具有诱导基因逆转和基因转化的活性。苯甲酰+哌啶磷-甲基混合物没有表现出任何基因毒性活性。乐果+重氮酮+氮磷甲酯的混合物具有遗传毒性，但各组分之间存在拮抗作用。添加S9线粒体后肝组分降低了单一和混合遗传毒性药物的活性。
Four organophosphorus pesticides (azinphos-methyl, diazinone, dimethoate, and pirimiphos-methyl), and one carbamate (benomyl) were tested for cytotoxicity, reverse mutation and gene conversion in Saccharomyces cerevisiae D7, with and without the S9 metabolic system. Furthermore, two mixtures of the above compounds, namely benomyl + pirimiphos-methyl (6/1 ratio) and dimethoate + diazinone + azinphos-methyl (10/4/6 ratio) were tested in the same experimental model. Azinphos-methyl, benomyl, and pirimiphos-methyl alone did not induce any genotoxic effect, whereas azinphos-methyl and diazinone were active in inducing reversion and gene conversion. The benomyl + pirimiphos-methyl mixture did not show any genotoxic activity. The dimethoate + diazinone + azimphosmethyl mixture was genotoxic, although an antagonistic effect between the components was observed. The addition of S9 post-mitochondrial liver fraction decreased the activity of both single and mixed genotoxic agents.