Development of microfluidic impedance cytometry enabling the quantification of specific membrane capacitance and cytoplasm conductivity from 100,000 single cells
Development of microfluidic impedance cytometry enabling the quantification of specific membrane capacitance and cytoplasm conductivity from 100,000 single cells
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微流控阻抗细胞仪的开发能够量化 100,000 个单细胞的特定膜电容和细胞质电导率。
DOI：
10.1016/j.bios.2018.04.015
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发表时间：
2018
影响因子：
12.6
通讯作者：
Huang Chengjun
中科院分区：
文献类型：
--
作者：
Zhao Yang;Wang Ke;Chen Deyong;Fan Beiyuan;Xu Ying;Ye Yifei;Wang Junbo;Chen Jian;Huang Chengjun
本文提出了一种新的具有交叉收缩微通道的微流控阻抗细胞术，能够表征细胞电标记物（例如，比膜电容（Csm）和细胞质电导率（σcy））。通过主缩窄通道连续抽吸单细胞，同时适当密封侧缩窄通道。建立了等效电路模型，并将测得的阻抗值转换为Csm和σcy。使用神经网络对不同的细胞群体进行分类，其中计算分类成功率。为了评估开发的技术，不同的肿瘤细胞系，上皮间充质转化对肿瘤细胞的影响进行了检查。H1299和HeLa细胞株的Csm和σ cy两个参数有显著性差异，两个参数联合使用的分类成功率为90.9%。A549细胞在上皮间质转化后Csm和σ cy均显著降低，分类成功率为76.5%。作为一种高通量的微流控阻抗细胞仪，该技术可以在单细胞分析中为流式细胞术增加一个新的无标记维度。
This paper presents a new microfluidic impedance cytometry with crossing constriction microchannels, enabling the characterization of cellular electrical markers (e.g., specific membrane capacitance (Csm) and cytoplasm conductivity (σcy)) in large cell populations (~ 100,000 cells) at a rate greater than 100 cells/s. Single cells were aspirated continuously through the major constriction channel with a proper sealing of the side constriction channel. An equivalent circuit model was developed and the measured impedance values were translated to Csmand σcy. Neural network was used to classify different cell populations where classification success rates were calculated. To evaluate the developed technique, different tumour cell lines, and the effects of epithelial-mesenchymal transitions on tumour cells were examined. Significant differences in both Csmand σcywere found for H1299 and HeLa cell lines with a classification success rate of 90.9% in combination of the two parameters. Meanwhile, tumour cells A549 showed significant decreases in both Csmand σcyafter epithelial-mesenchymal transitions with a classification success rate of 76.5%. As a high-throughput microfluidic impedance cytometry, this technique can add a new marker-free dimension to flow cytometry in single-cell analysis.