Breeding structure of Glossina pallidipes populations evaluated by mitochondrial variation.
Breeding structure of Glossina pallidipes populations evaluated by mitochondrial variation.
复制标题
DOI：
10.1093/jhered/90.6.635
复制
发表时间：
1999-11
期刊：
影响因子：
--
通讯作者：
E. Krafsur;DL Wohlford
中科院分区：
文献类型：
--
作者：
E. Krafsur;DL Wohlford
对来自津巴布韦、莫桑比克、肯尼亚和埃塞俄比亚的6个采采蝇自然种群的4个位点的线粒体DNA多样性进行了研究。单位点多样性在12S时为0.39 ~ COII时为0.65。共发现32个单倍型，平均每个位点6.4 +/- 2.9个。为了研究繁殖结构，对来自约1,611,000 km2的18个种群和3个实验室培养的COII和16S2两个位点进行了多样性评估。在2个位点检测到26个单倍型，自然种群的平均单倍型多样性为0.63。在埃塞俄比亚和肯尼亚人口内部和人口之间发现了高度的人口细分。津巴布韦和赞比亚种群表现出比北方种群少得多的变异和分化。莫桑比克的一个种群显示出高水平的单倍型变异，并且与肯尼亚东部（距离其北部约1700公里）的种群最接近。单倍型频率方差分析表明，居群内居群占总居群的51.5%，居群内居群占总居群的13%，居群间居群占总居群的35.5%。Wright的FST为0.485，Nei的GST为0.33，Weir和Cunningham的θ = 0.45。生态学资料表明，苍白棘球蚴具有高度的柔韧性。大量的遗传分化可以用19世纪末和20世纪初牛瘟大流行期间在分散的、孤立的种群中发生的遗传漂变来解释。
Mitochondrial DNA diversity was studied at four loci in six natural populations of the tsetse fly Glossina pallidipes from Zimbabwe, Mozambique, Kenya, and Ethiopia. Single-locus diversity varied from 0.39 at 12S to 0.65 at COII. A total of 32 haplotypes was found with a mean of 6.4 +/- 2.9 per locus. To study breeding structure, diversity at two loci, COII and 16S2, was evaluated in 18 populations sampled from an area of approximately 1,611,000 km2 and in three laboratory cultures. Twenty-six haplotypes were detected at the two loci and mean haplotype diversity over all natural populations was 0.63. A high degree of population subdivision was detected within and among the Ethiopian and Kenya populations. The Zimbabwe and Zambia populations showed much less variation and differentiation than the northern populations. A population in Mozambique showed high levels of haplotype variation and affinities closest to populations in eastern Kenya, some 1700 km to the north. Analysis of variance of haplotype frequencies showed that 51.5% of the total lay within populations, 13% among populations within five nested groups, and 35.5% among the five groups. Wright's FST was 0.485, Nei's GST was 0.33, and Weir and Cunningham's theta = 0.45. Ecological data show that G. pallidipes is highly vagile. The large amount of genetic differentiation may be explained by genetic drift that occurred in scattered, relict populations during the rinderpest panzootic of the late 19th and early 20th centuries.