SERUM ESTERASES .1. 2 TYPES OF ESTERASE (A AND B) HYDROLYSING PARA-NITROPHENYL ACETATE, PROPIONATE AND BUTYRATE, AND A METHOD FOR THEIR DETERMINATION
SERUM ESTERASES .1. 2 TYPES OF ESTERASE (A AND B) HYDROLYSING PARA-NITROPHENYL ACETATE, PROPIONATE AND BUTYRATE, AND A METHOD FOR THEIR DETERMINATION
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DOI：
10.1042/bj0530110
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发表时间：
1953-01-01
影响因子：
4.1
通讯作者：
ALDRIDGE, WN
中科院分区：
文献类型：
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作者：
ALDRIDGE, WN
用12种血清与不同浓度的血清孵育30分钟后，测定了对硝基苯乙酸酯、丙酸酯和丁酸酯的水解作用。E 600（对硝基苯基磷酸酯）和其他抑制剂。血清酯酶可分为两种不同的类型：A，不受E 600的抑制，其水解对硝基苯基丁酸酯的速度快于乙酸酯; B，受10-7 e E 600的抑制，其水解丁酸酯的速度与乙酸酯相同或更快。乙酰丝氨酸对酶活性无影响，而镍、铜、对氯汞苯甲酸和氟化物对酶活性有抑制作用。大型amts。A型见于雪貂、山羊、兔和绵羊血清，B型见于豚鼠和大鼠血清。人血清和牛血清只含有中等量的amts。A型的。
The hydrolysis of p-nitrophenyl acetate, propionate and butyrate by 12 sera was tested after 30 min. incubation of the sera with varying concns. of E 600 (p-nitrophenyl phosphate) and other inhibitors. The serum esterases may be separated into 2 distinct types; A, which is not inhibited by E 600 which hydrolyses p-nitrophenyl butyrate faster than acetate, and B, which is inhibited by 10-7 e E 600 and hydrolyzes the butyrate at the same rate or faster than acetate. Eserine has no effect on the activity, but Ni, Cu, p-chloromercuribenzoic acid and fluoride are inhibitory. Large amts. of type A are found in ferret, goat, rabbit and sheep sera, of type B in guinea pig and rat sera. Human and beef sera contain only moderate amts. of type A.