Differential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes.
Differential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes.
复制标题
DOI：
10.1016/j.talanta.2005.12.041
复制
发表时间：
2006-07
期刊：
影响因子：
6.1
通讯作者：
Minli Yang;Zhanjun Zhang;Zhong-bo Hu;Jinghong Li
中科院分区：
文献类型：
--
作者：
Minli Yang;Zhanjun Zhang;Zhong-bo Hu;Jinghong Li
金属硫蛋白（metallothionein，MT）作为金属蛋白的代表，在生物和环境中具有重要的作用，如对某些金属的代谢和解毒等。在pH 2-5.5范围内，产生两个界限分明的阳极峰，这两个峰是MT固有的Zn ~（2+）和Cd ~（2+）。MT固有的Cd 2+的DPASV峰电流与MT浓度的校准曲线显示出良好的线性关系，MT的检测限为3.86×10− 8 mol/L。作为一种无毒的优良电极材料，BiFE在金属硫蛋白的检测中表现出良好的性能，有望在其他金属蛋白的研究中得到进一步的应用。
As a representation of metalloproteins, metallothionein (MT), which plays important biological and environmental roles such as in the metabolism and detoxification of some metals, was detected at bismuth film electrode (BiFE) by differential pulse anodic stripping voltammetry (DPASV). In pH 2–5.5, two well-defined anodic peaks were produced and attributed to the Zn2+and Cd2+inherent to MT. The calibration plot of DPASV peak currents for Cd2+inherent to MT versus MT concentrations showed a good linearity with a detection limit of 3.86×10−8mol/L for MT. As a non-toxic excellent electrode material, BiFE shows good performance for detecting MT, and is expected to find further applications in the studies of many other metalloproteins.