Genes commonly regulated by water-deficit stress in Arabidopsis thaliana
Genes commonly regulated by water-deficit stress in Arabidopsis thaliana
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DOI：
10.1093/jxb/erh270
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发表时间：
2004-11-01
影响因子：
6.9
通讯作者：
Bray, EA
中科院分区：
文献类型：
--
作者：
Bray, EA
细胞缺水胁迫触发了基因表达的许多变化，这些变化可以用来定义植物对环境条件的反应。微阵列技术可以同时研究数千个基因的表达模式，从而全面了解细胞中存在的响应缺水胁迫的RNA的类型和数量。在三个不同的实验中对特定基因的表达进行了比较，这些实验旨在了解基因表达在缺水胁迫下的变化。令人惊讶的是，有一组相对较小的基因通常被诱导或抑制。共有27个基因被共同诱导，3个基因被共同抑制；在所有三个实验中，分别有1.4%和0.2%的基因被分析为共同的。被诱导的基因分为六个不同的功能类别：新陈代谢、运输、信号、转录、亲水蛋白和未知。这三个普遍被抑制的基因表明，基因表达的抑制支持了人们经常观察到的对缺水胁迫的反应，即生长下降。对涉及细胞壁新陈代谢的基因的更详细分析表明，促进细胞扩张的基因的表达在全球范围内都有所下降。
Cellular water-deficit stress triggers many changes in gene expression which can be used to define the response of a plant to an environmental condition. Microarray technology permits the study of expression patterns of thousands of genes simultaneously, permitting a comprehensive understanding of the types and quantities of RNAs that are present in a cell in response to water-deficit stress. The expression of specific genes was compared in three different experiments designed to understand changes in gene expression in response to water-deficit stress. Surprisingly, there was a relatively small set of genes that were commonly induced or repressed. There were 27 genes commonly induced and three commonly repressed; 1.4% and 0.2% of the genes analysed in common to all three experiments. The induced genes fell into six different functional categories: metabolism, transport, signalling, transcription, hydrophilic proteins, and unknown. The three commonly repressed genes indicated that repression of gene expression supported a frequently observed response to water-deficit stress, decreased growth. A more detailed analysis of genes involved in cell wall metabolism, indicated that there was a global decrease in expression of genes that promote cell expansion.