Glutamate in the medium of Lactiplantibacillus plantarum FL-664 affects the production of IL-12(p40) on murine spleen cells
Glutamate in the medium of Lactiplantibacillus plantarum FL-664 affects the production of IL-12(p40) on murine spleen cells
复制标题
植物乳杆菌 FL-664 培养基中的谷氨酸影响小鼠脾细胞 IL-12(p40) 的产生
DOI：
10.1093/bbb/zbac006
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Nomoto Koji
中科院分区：
文献类型：
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作者：
Kawamoto-Miyamoto Noriko;Hosoda Hiroshi;Miyoshi Kazuyuki;Nomoto Koji
乳酸菌(LAB)因其对天然免疫功能的影响而备受关注，因此需要在保持其功能的同时开发一种有效的培养方法。在本研究中，我们首先比较了食品级(FG)和标准LAB培养液培养的LAB的生长和功能，发现FG培养的LAB细胞体积小，产量高，体外诱导小鼠脾细胞产生IL-12(P40)的能力更强。此外，培养液中谷氨酸浓度越高，菌体越小，产量越高，诱导产生IL-12的能力越强。在培养基中添加谷氨酸会改变LAB的大小，并影响其诱导IL-12(P40)产生的能力。综上所述，调节谷氨酸浓度对功能实验室的高效培养具有重要意义。
Lactic acid bacteria (LAB) have been attracting attention for their effects on innate immunity, and therefore, it is required to develop an efficient culturing method while maintaining their functionality. In this study, first, we compared the growth and functionality of LAB cultured on food grade (FG) medium with those on standard LAB medium and found that LAB cultured in the FG medium were smaller in cell size with high yield and had a higher ability to induce IL-12(p40) production by murine spleen cells in vitro. Moreover, the higher the glutamate concentration in the medium, the smaller the cell size, and the higher the yield and the higher the ability to induce IL-12 production. Addition of glutamate to the culture medium changes the size of LAB and affects their ability to induce IL-12(p40) production. In conclusion, regulating the concentration of glutamate would be important in the efficient culturing of functional LAB.