The Arabidopsis Hypoxia Inducible AtR8 Long Non-Coding RNA also Contributes to Plant Defense and Root Elongation Coordinating with WRKY Genes under Low Levels of Salicylic Acid
The Arabidopsis Hypoxia Inducible AtR8 Long Non-Coding RNA also Contributes to Plant Defense and Root Elongation Coordinating with WRKY Genes under Low Levels of Salicylic Acid
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DOI：
10.3390/ncrna6010008
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发表时间：
2020-03-01
期刊：
影响因子：
4.3
通讯作者：
Yukawa, Yasushi
中科院分区：
文献类型：
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作者：
Li, Shuang;Nayar, Saraswati;Yukawa, Yasushi
AtR8长链非编码RNA（lncRNA）先前在开花植物拟南芥中被鉴定出来，它是一种由RNA聚合酶III转录的、含量丰富的长链非编码RNA，长度约为260个核苷酸。AtR8 lncRNA的积累对根部的缺氧胁迫和水杨酸（SA）处理有响应，但其功能尚未明确。在本研究中，对atr8突变体和野生型拟南芥进行的微阵列分析表明，AtR8 lncRNA与防御反应密切相关。当植物暴露于外源低浓度SA（20微摩尔）、感染丁香假单胞菌或处于发育早期时，AtR8的积累与两个WRKY基因（WRKY53/WRKY70）的积累呈负相关。在种子萌发5天后观察到AtR8积累量最高，此时检测不到WRKY53或WRKY70的信使核糖核酸（mRNA）。低水平SA的存在导致atr8幼苗根长显著缩短，而wrky53和wrky70突变体则表现出相反的表型。综上所述，AtR8 lncRNA参与了与SA反应相关的、不依赖病程相关蛋白1（PR - 1）的防御反应和根伸长过程。AtR8 lncRNA与WRKY53/WRKY70的相互调控是由病程相关基因非表达子1（NPR1）介导的。
AtR8 lncRNA was previously identified in the flowering plant Arabidopsis thaliana as an abundant Pol III-transcribed long non-coding RNA (lncRNA) of approximately 260 nt. AtR8 lncRNA accumulation is responsive to hypoxic stress and salicylic acid (SA) treatment in roots, but its function has not yet been identified. In this study, microarray analysis of an atr8 mutant and wild-type Arabidopsis indicated a strong association of AtR8 lncRNA with the defense response. AtR8 accumulation exhibited an inverse correlation with an accumulation of two WRKY genes (WRKY53/WRKY70) when plants were exposed to exogenous low SA concentrations (20 mu M), infected with Pseudomonas syringae, or in the early stage of development. The highest AtR8 accumulation was observed 5 days after germination, at which time no WRKY53 or WRKY70 mRNA was detectable. The presence of low levels of SA resulted in a significant reduction of root length in atr8 seedlings, whereas wrky53 and wrky70 mutants exhibited the opposite phenotype. Taken together, AtR8 lncRNA participates in Pathogenesis-Related Proteins 1 (PR-1)-independent defense and root elongation, which are related to the SA response. The mutual regulation of AtR8 lncRNA and WRKY53/WRKY70 is mediated by Nonexpressor of Pathogenesis-Related Gene 1 (NPR1).