A NEW CONTINUOUS CELL LINE FROM LARVAE OF THE MOSQUITO AEDES ALBOPICTUS (DIPTERA, CULICIDAE)
A NEW CONTINUOUS CELL LINE FROM LARVAE OF THE MOSQUITO AEDES ALBOPICTUS (DIPTERA, CULICIDAE)
复制标题
白纹伊蚊（双翅目，蚊科）幼虫的新连续细胞系
DOI：
10.2220/biomedres.2.599
复制
发表时间：
1981
影响因子：
1.2
通讯作者：
J. Mitsuhashi
中科院分区：
文献类型：
--
作者：
J. Mitsuhashi
以伊蚊（Aedes aibopicms）一龄幼虫为材料，建立了连续细胞系。原代培养是由幼虫体块制备的。各种形状的细胞从外植的幼虫碎片中迁移出来，在培养容器的玻璃表面繁殖。培养53天后进行第一次传代培养，625天后进行100代传代。建立的细胞系已被命名为nias - aal -2。细胞系染色体数模式为6 （2n）。在25℃条件下，种群倍增时间约为30小时。细胞可在5℃下保存约2个月。这些细胞比其他蚊子细胞系更活跃地消耗天冬氨酸和谷氨酸。培养物明显不受支原体和病毒的污染。细胞对Chilo虹彩病毒（虹彩病毒科）敏感。该细胞系可在无血清培养基中连续培养。
A continuous cell line was established from the first instar larvae of a mosquito, Aedes aibopicms. Primary cultures were prepared from pieces of larval bodies. Cells of various shapes migrated from the explanted larval fragments and multiplied on the glass surface of the culture vessel. The first subculture was made 53 days after the culture was set up, and 100 passages were made during the following 625 days. The established cell line has been designated as NIAS-AeAl-2. The mode of chromosome number of the cell line was 6 (2n). Population doubling time was about 30 hr at 25°C. Cells could be stored at 5°C for about 2 months. The cells consumed aspartic acid and glutamic acid more actively than other mosquito cell lines. Cultures were apparently free from contamination by mycoplasmas and viruses. The cells were susceptible to Chilo iridescent virus (Iridoviridae). The cell line could be cultured continuously in serum-free culture media.